Protein lysine methyltransferase G9a acts on non-histone targets (original) (raw)

Nature Chemical Biology volume 4, pages 344–346 (2008)Cite this article

Abstract

By methylation of peptide arrays, we determined the specificity profile of the protein methyltransferase G9a. We show that it mostly recognizes an Arg-Lys sequence and that its activity is inhibited by methylation of the arginine residue. Using the specificity profile, we identified new non-histone protein targets of G9a, including CDYL1, WIZ, ACINUS and G9a (automethylation), as well as peptides derived from CSB. We demonstrate potential downstream signaling pathways for methylation of non-histone proteins.

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Acknowledgements

The US National Institutes of Health (GM068680) and the Bundesministerium für Bildung und Forschung Biofuture program have supported this work. We thank M.T. Bedford (University of Texas M.D. Anderson Cancer Center) for the GST-HP1β expression construct and M. Yoshida (RIKEN) for technical advice on anti-pan methyllysine antibodies. Technical assistance by M. Schwerdtfeger (Jacobs University Bremen) is gratefully acknowledged.

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Authors and Affiliations

  1. Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, Bremen, 28759, Germany
    Philipp Rathert, Arunkumar Dhayalan, Raluca Tamas, Renata Jurkowska & Albert Jeltsch
  2. Research Center for Infectious Diseases, Institute for Virus Research and Graduate School of Biostudies, Kyoto University, 53 Shogoin, Kawara-cho, Sakyo-ku, Kyoto, 606-8507, Kyoto, Japan
    Marie Murakami & Yoichi Shinkai
  3. Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, 30322, Georgia, USA
    Xing Zhang & Xiaodong Cheng
  4. Biochemistry and Cell Biology Program, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, Bremen, 28759, Germany
    Raluca Tamas
  5. R&D Division, Advanced Life Science Institute Inc., 2-10-23, Maruyamadai, Wako, 351-0112, Saitama, Japan
    Yasuhiko Komatsu

Authors

  1. Philipp Rathert
  2. Arunkumar Dhayalan
  3. Marie Murakami
  4. Xing Zhang
  5. Raluca Tamas
  6. Renata Jurkowska
  7. Yasuhiko Komatsu
  8. Yoichi Shinkai
  9. Xiaodong Cheng
  10. Albert Jeltsch

Corresponding author

Correspondence toAlbert Jeltsch.

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Rathert, P., Dhayalan, A., Murakami, M. et al. Protein lysine methyltransferase G9a acts on non-histone targets.Nat Chem Biol 4, 344–346 (2008). https://doi.org/10.1038/nchembio.88

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