Chromosome specific paints from a high resolution flow karyotype of the mouse (original) (raw)
- Article
- Published: 01 April 1995
- Helen Impey1,
- Amanda Heppell-Parton1,
- Cordelia Langford2 nAff4,
- Charles Tease3,
- Nicholas Lowe1,
- David Bailey2,
- Malcolm Ferguson-Smith2 &
- …
- Nigel Carter2 nAff4
Nature Genetics volume 9, pages 369–375 (1995)Cite this article
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Abstract
Chromosomes from antigen stimulated B–cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus–specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced.
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Author notes
- Cordelia Langford & Nigel Carter
Present address: The Sanger Centre, Hinxton Hall, Hinxton, Cambridge, CB10 IRQ, UK
Authors and Affiliations
- Clinical Oncology & MRC Radiotherapeutics Unit, MRC Centre, Hills Road, Cambridge, CB2 2QH, UK
Pamela Rabbitts, Helen Impey, Amanda Heppell-Parton & Nicholas Lowe - University Dept of Pathology, Tennis Court Road, Cambridge, CB2 1QP, UK
Cordelia Langford, David Bailey, Malcolm Ferguson-Smith & Nigel Carter - MRC Radiobiology Unit, Chilton, Didcot, Oxfordshire, OX11 0RD, UK
Charles Tease
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Rabbitts, P., Impey, H., Heppell-Parton, A. et al. Chromosome specific paints from a high resolution flow karyotype of the mouse.Nat Genet 9, 369–375 (1995). https://doi.org/10.1038/ng0495-369
- Received: 17 November 1994
- Accepted: 17 January 1995
- Issue Date: 01 April 1995
- DOI: https://doi.org/10.1038/ng0495-369