Splinkerette PCR for more efficient characterization of gene trap events (original) (raw)

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In the version of this article initially published, the second author (Jens Hansen) should have been listed as an equal contributor with the first author. The last two authors (Harald von Melchner and Patricia Ruiz Noppinger) should have been listed as corresponding authors. The error has been corrected in the HTML and PDF versions of the article.

References

  1. International Mouse Knockout Consortium, Collins, F.S., Rossant, J. & Wurst, W. Cell 128, 9–13 (2007).
    Article CAS Google Scholar
  2. Stryke, D. et al. Nucleic Acids Res. 31, 278–281 (2003).
    Article CAS Google Scholar
  3. Schnutgen, F. et al. Proc. Natl. Acad. Sci. USA 102, 7221–7226 (2005).
    Article Google Scholar
  4. Hansen, J. et al. Proc. Natl. Acad. Sci. USA 100, 9918–9922 (2003).
    Article CAS Google Scholar
  5. Zambrowicz, B.P. et al. Proc. Natl. Acad. Sci. USA 100, 14109–14114 (2003).
    Article Google Scholar
  6. Devon, R.S., Porteous, D.J. & Brookes, A.J. Nucleic Acids Res. 23, 1644–1645 (1995).
    Article CAS Google Scholar
  7. Friedrich, G. & Soriano, P. Genes Dev. 5, 1513–1523 (1991).
    Article CAS Google Scholar
  8. Skarnes, W.C., Moss, J.E., Hurtley, S.M. & Beddington, R.S. Proc. Natl. Acad. Sci. USA 92, 6592–6596 (1995).
    Article CAS Google Scholar
  9. Yenofsky, R.L., Fine, M. & Pellow, J.W. Proc. Natl. Acad. Sci. USA 87, 3435–3439 (1990).
    Article CAS Google Scholar
  10. Skarnes, W.C. et al. Nat. Genet. 36, 543–544 (2004).
    Article CAS Google Scholar

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Acknowledgements

We thank T. Cox and L. von Melchner for helpful discussions and for review of the final manuscript. We also thank C. Werner, B. Thalke, M. Hollatz, D. German, A.-T. Tieu and S. Buchner for technical assistance. This work was supported by grants from the Bundesministerium für Bildung und Forschung (BMBF) to the German Gene Trap Consortium (GGTC) and by grants from the European Union to the European Conditional Mouse Mutagenesis (EUCOMM) program.

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Author notes

  1. Carsten Horn and Jens Hansen: These authors contributed equally to this work.
  2. Harald von Melchner and Patricia Ruiz Noppinger: These authors contributed equally to this work. melchner@em.uni-frankfurt.de or ruiz@molgen.mpg.de

Authors and Affiliations

  1. Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, 14195, Germany
    Carsten Horn, Markus Irgang & Patricia Ruiz Noppinger
  2. Institute of Developmental Genetics, GSF-National Research Center for Environment and Health, Neuherberg, 85764, Germany
    Jens Hansen, Claudia Seisenberger, Thomas Floss & Wolfgang Wurst
  3. Department for Molecular Hematology, University of Frankfurt Medical School, Frankfurt am Main, 60590, Germany
    Frank Schnütgen, Silke De-Zolt & Harald von Melchner
  4. Center for Cardiovascular Research, Charité–Universitätsmedizin, Berlin, 10115, Germany
    Patricia Ruiz Noppinger
  5. The German Gene Trap Consortium,
    Carsten Horn, Jens Hansen, Frank Schnütgen, Claudia Seisenberger, Thomas Floss, Markus Irgang, Silke De-Zolt, Wolfgang Wurst, Harald von Melchner & Patricia Ruiz Noppinger

Authors

  1. Carsten Horn
  2. Jens Hansen
  3. Frank Schnütgen
  4. Claudia Seisenberger
  5. Thomas Floss
  6. Markus Irgang
  7. Silke De-Zolt
  8. Wolfgang Wurst
  9. Harald von Melchner
  10. Patricia Ruiz Noppinger

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Competing interests

The authors declare no competing financial interests.

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Horn, C., Hansen, J., Schnütgen, F. et al. Splinkerette PCR for more efficient characterization of gene trap events.Nat Genet 39, 933–934 (2007). https://doi.org/10.1038/ng0807-933

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