Probing tumor phenotypes using stable and regulated synthetic microRNA precursors (original) (raw)

References

  1. Brummelkamp, T.R., Bernards, R. & Agami, R. A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550–553 (2002).
    Article CAS Google Scholar
  2. Paddison, P.J., Caudy, A.A., Bernstein, E., Hannon, G.J. & Conklin, D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948–958 (2002).
    Article CAS Google Scholar
  3. Xia, H., Mao, Q., Paulson, H.L. & Davidson, B.L. siRNA-mediated gene silencing in vitro and in vivo. Nat. Biotechnol. 20, 1006–1010 (2002).
    Article CAS Google Scholar
  4. Unwalla, H.J. et al. Negative feedback inhibition of HIV-1 by TAT-inducible expression of siRNA. Nat. Biotechnol. 22, 1573–1578 (2004).
    Article CAS Google Scholar
  5. Ling, X. & Li, F. Silencing of antiapoptotic survivin gene by multiple approaches of RNA interference technology. Biotechniques 36, 450–454, 456–460 (2004).
    Article CAS Google Scholar
  6. Song, J. et al. Gene silencing in androgen-responsive prostate cancer cells from the tissue-specific prostate-specific antigen promoter. Cancer Res. 64, 7661–7663 (2004).
    Article CAS Google Scholar
  7. Zeng, Y., Wagner, E.J. & Cullen, B.R. Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol. Cell 9, 1327–1333 (2002).
    Article CAS Google Scholar
  8. Zeng, Y., Cai, X. & Cullen, B.R. Use of RNA polymerase II to transcribe artificial microRNAs. Methods Enzymol. 392, 371–380 (2005).
    Article CAS Google Scholar
  9. Boden, D. et al. Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins. Nucleic Acids Res. 32, 1154–1158 (2004).
    Article CAS Google Scholar
  10. Silva, J.M. et al. Second-generation shRNA libraries covering the mouse and human genomes. Nat. Genet. advance online publication xx xxx 2005 (10.1038/ngXXX). [date and doi for hannon].
  11. Hemann, M.T. et al. An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo. Nat. Genet. 33, 396–400 (2003).
    Article CAS Google Scholar
  12. Lee, Y. et al. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 23, 4051–4060 (2004).
    Article CAS Google Scholar
  13. Cai, X., Hagedorn, C.H. & Cullen, B.R. Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10, 1957–1966 (2004).
    Article CAS Google Scholar
  14. Rubinson, D.A. et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33, 401–406 (2003).
    Article CAS Google Scholar
  15. Egle, A., Harris, A.W., Bouillet, P. & Cory, S. Bim is a suppressor of Myc-induced mouse B cell leukemia. Proc. Natl. Acad. Sci. USA 101, 6164–6169 (2004).
    Article CAS Google Scholar
  16. Schmitt, C.A., McCurrach, M.E., de Stanchina, E., Wallace-Brodeur, R.R. & Lowe, S.W. INK4a/ARF mutations accelerate lymphomagenesis and promote chemoresistance by disabling p53. Genes Dev. 13, 2670–2677 (1999).
    Article CAS Google Scholar
  17. van de Wetering, M. et al. Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep. 4, 609–615 (2003).
    Article CAS Google Scholar
  18. Miyagishi, M., Sumimoto, H., Miyoshi, H., Kawakami, Y. & Taira, K. Optimization of an siRNA-expression system with an improved hairpin and its significant suppressive effects in mammalian cells. J. Gene Med. 6, 715–723 (2004).
    Article CAS Google Scholar
  19. Czauderna, F. et al. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res. 31, e127 (2003).
    Article Google Scholar
  20. Chen, Y., Stamatoyannopoulos, G. & Song, C.Z. Down-regulation of CXCR4 by inducible small interfering RNA inhibits breast cancer cell invasion in vitro. Cancer Res. 63, 4801–4804 (2003).
    CAS PubMed Google Scholar
  21. Wiznerowicz, M. & Trono, D. Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. J. Virol. 77, 8957–8961 (2003).
    Article CAS Google Scholar
  22. Matsukura, S., Jones, P.A. & Takai, D. Establishment of conditional vectors for hairpin siRNA knockdowns. Nucleic Acids Res. 31, e77 (2003).
    Article Google Scholar
  23. Hosono, T. et al. Adenovirus vector-mediated doxycycline-inducible RNA interference. Hum. Gene Ther. 15, 813–819 (2004).
    Article CAS Google Scholar
  24. Gupta, S., Schoer, R.A., Egan, J.E., Hannon, G.J. & Mittal, V. Inducible, reversible, and stable RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 101, 1927–1932 (2004).
    Article CAS Google Scholar
  25. Gossen, M. & Bujard, H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89, 5547–5551 (1992).
    Article CAS Google Scholar
  26. Gossen, M. et al. Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766–1769 (1995).
    Article CAS Google Scholar
  27. Serrano, M., Lin, A.W., McCurrach, M.E., Beach, D. & Lowe, S.W. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell 88, 593–602 (1997).
    Article CAS Google Scholar
  28. Dirac, A.M. & Bernards, R. Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53. J. Biol. Chem. 278, 11731–11734 (2003).
    Article CAS Google Scholar
  29. Felsher, D.W. Reversibility of oncogene-induced cancer. Curr. Opin. Genet. Dev. 14, 37–42 (2004).
    Article CAS Google Scholar
  30. Harvey, M. et al. In vitro growth characteristics of embryo fibroblasts isolated from p53-deficient mice. Oncogene 8, 2457–2467 (1993).
    CAS PubMed Google Scholar
  31. Stegmeier, F., Hu, G., Rickles, R., Hannon, G.J. & Elledge, S.J. A lentiviral microRNA-based system for single copy Pol II regulated RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 102, 13212–13217 (2005).
    Article CAS Google Scholar
  32. Paddison, P.J. et al. Cloning of short hairpin RNAs for gene knockdown in mammalian cells. Nat. Methods 1, 163–167 (2004).
    Article CAS Google Scholar
  33. Schmitt, C.A., Rosenthal, C.T. & Lowe, S.W. Genetic analysis of chemoresistance in primary murine lymphomas. Nat. Med. 6, 1029–1035 (2000).
    Article CAS Google Scholar
  34. Schmitt, C.A. et al. A senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy. Cell 109, 335–346 (2002).
    Article CAS Google Scholar
  35. Zuker, M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res. 31, 3406–3415 (2003).
    Article CAS Google Scholar

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