Assembly of microarrays for genome-wide measurement of DNA copy number (original) (raw)

Nature Genetics volume 29, pages 263–264 (2001) Cite this article

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05–0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

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Acknowledgements

We thank J. Flint, D. Ledbetter, C. Lese and Vysis, Inc. for telomere clones and clones containing certain named genes. This work was supported by NIH grants CA80314, CA83040, CA84118, HD17665 and CA58207, by California BCRP grant 2RB-0225 and by Vysis, Inc.

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Authors and Affiliations

  1. Comprehensive Cancer Center, University of California San Francisco, San Francisco, 94143, California
    Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Nils Brown, Greg Hamilton, Anna Katherine Hindle, Bing Huey, Karen Kimura, Sindy Law, Ken Myambo, Joel Palmer, Bauke Ylstra, Jingzhu Pearl Yue, Joe W. Gray, Ajay N. Jain, Daniel Pinkel & Donna G. Albertson
  2. Cancer Research Institute, University of California San Francisco, San Francisco, 94143, California
    Antoine M. Snijders, Stephanie Blackwood, Anna Katherine Hindle, Sindy Law, Joel Palmer, Bauke Ylstra, Ajay N. Jain & Donna G. Albertson
  3. Department of Laboratory Medicine, University of California San Francisco, San Francisco, 94143, California
    Joe W. Gray, Ajay N. Jain, Daniel Pinkel & Donna G. Albertson
  4. Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, 14263, New York
    Norma Nowak & Jeffrey Conroy

Authors

  1. Antoine M. Snijders
  2. Norma Nowak
  3. Richard Segraves
  4. Stephanie Blackwood
  5. Nils Brown
  6. Jeffrey Conroy
  7. Greg Hamilton
  8. Anna Katherine Hindle
  9. Bing Huey
  10. Karen Kimura
  11. Sindy Law
  12. Ken Myambo
  13. Joel Palmer
  14. Bauke Ylstra
  15. Jingzhu Pearl Yue
  16. Joe W. Gray
  17. Ajay N. Jain
  18. Daniel Pinkel
  19. Donna G. Albertson

Corresponding author

Correspondence toDonna G. Albertson.

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Snijders, A., Nowak, N., Segraves, R. et al. Assembly of microarrays for genome-wide measurement of DNA copy number.Nat Genet 29, 263–264 (2001). https://doi.org/10.1038/ng754

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