Truncating mutations of RB1CC1 in human breast cancer (original) (raw)
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- Published: 17 June 2002
Nature Genetics volume 31, pages 285–288 (2002)Cite this article
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Abstract
The protein RB1CC1 (retinoblastoma 1 (RB1)-inducible coiled-coil 1) has been identified as a key regulator of the tumor-suppressor gene RB1 (ref. 1). RB1CC1 is localized in the nucleus and has been proposed to be a transcription factor because of its nuclear localization signal, leucine zipper motif and coiled-coil structure1,2. The gene RB1CC1 is localized to a region of chromosome 8q11 (ref. 2) containing several loci of putative tumor-suppressor genes3,4; however, its role in human cancers remains to be determined. Here we report that 20% (7 of 35) of primary breast cancers examined contained mutations in RB1CC1, including nine large interstitial deletions predicted to yield markedly truncated RB1CC1 proteins. Wildtype RB1CC1 and RB1 were absent or significantly less abundant than normal in the seven cancers with mutations in RB1CC1, but were abundant in cancers without such mutations. In all seven cancers, both RB1CC1 alleles were inactivated; two showed compound heterozygous deletions. Thus, RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor-suppressor gene.
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References
- Chano, T. et al. Identification of RB1CC1, a novel gene that can induce RB1 in various human cells. Oncogene 21, 1295–1298 (2002).
Article CAS Google Scholar - Chano, T. et al. Isolation, characterization and mapping of the mouse and human RB1CC1 genes. Gene (in press).
- Dahiya, R., Perinchery, G., Deng, G. & Lee, C. Multiple sites of loss of heterozygosity on chromosome 8 in human breast cancer has differential correlation with clinical parameters. Int. J. Oncol. 12, 811–816 (1998).
CAS PubMed Google Scholar - Perinchery, G. et al. Loss of two new loci on chromosome 8 (8p23 and 8q12-13) in human prostate cancer. Int. J. Oncol. 14, 495–500 (1999).
CAS PubMed Google Scholar - Kaelin, W.G. Jr. Functions of the retinoblastoma protein. BioEssays 21, 950–958 (1999).
Article Google Scholar - Kamb, A. Cell cycle regulators and cancers. Trends Genet. 11, 136–140 (1995).
Article CAS Google Scholar - Taya, Y. RB kinases and RB-binding proteins: new points of view. Trends Biol. Sci. 22, 14–17 (1997).
Article CAS Google Scholar - T'Ang, A., Varley, J.M., Chakraborty, S., Murphree, A.L. & Fung, Y.K. Structural rearrangement of the human retinoblastoma gene in human breast carcinoma. Science 242, 263–266 (1988).
Article CAS Google Scholar - Bieche, I. & Lidereau, R. Loss of heterozygosity at 13q14 correlate with RB1 gene underexpression in human breast cancer. Mol. Carcinog. 29, 151–158 (2000).
Article CAS Google Scholar - Thorlacious, S., Jonasdottir, O. & Eyfjord, J.E. Loss of heterozygosity at selective sites on chromosomes 13 and 17 in human breast carcinoma. Anticancer Res. 11, 1501–1507 (1991).
Google Scholar - Lemoine, N.R. Molecular biology of breast cancer. Ann. Oncol. 5 (Suppl. 4), 31–37 (1994).
Article Google Scholar - Varley, J.M. et al. The retinoblastoma gene is frequently altered leading to loss of expression in primary breast tumors. Oncogene 4, 725–729 (1989).
CAS PubMed Google Scholar - Wadayama, B. et al. The spectrum of the retinoblastoma gene in osteosarcomas. Cancer Res. 54, 3042–3048 (1994).
CAS PubMed Google Scholar
Acknowledgements
We thank A. Mabuchi for computational searches and H. Honjo, H. Chen, N. Takashima, M. Sugimoto, K. Kobayashi and J. Shimizu for experimental assistance. This study was partially supported by grant-in-aids for Japan Society for the Promotion of Science Fellows, Scientific Research (B), The Ministry of Education, Science, Sports and Culture of Japan, and a grant from the Japan Orthopaedics and Traumatology Foundation.
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Authors and Affiliations
- Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Seta, Otsu, 520-2192, Shiga, Japan
Tokuhiro Chano & Hidetoshi Okabe - Department of Surgery II, Shiga University of Medical Science, Seta, Otsu, 520-2192, Shiga, Japan
Keiichi Kontani & Koji Teramoto - Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN,, Tokyo, 108-8639, Japan
Shiro Ikegawa
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- Tokuhiro Chano
You can also search for this author inPubMed Google Scholar - Keiichi Kontani
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Correspondence toTokuhiro Chano.
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Chano, T., Kontani, K., Teramoto, K. et al. Truncating mutations of RB1CC1 in human breast cancer.Nat Genet 31, 285–288 (2002). https://doi.org/10.1038/ng911
- Received: 18 December 2001
- Accepted: 09 May 2002
- Published: 17 June 2002
- Issue Date: July 2002
- DOI: https://doi.org/10.1038/ng911