Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis (original) (raw)

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Acknowledgements

DH4 monoclonal antibody was a gift from A.M. Krensky (Stanford University). We thank H.-J. Lin, S.-J. Li, K.-H. Chen, L.-H. Lu of the Institute of Biomedical Sciences, Academia Sinica and members of the National Microarray & Gene Expression Analysis Core Facility, National Yang-Ming University, Taiwan, for excellent technical assistance. This work was supported by grants from the National Science Council, Taiwan (NSC95-2314-B-182A-048, 95-2314-B-010-096, 96-2320-B-010-021-MY2 and 96-2628-B-182A-065-MY2), Chang-Gung Memorial Hospital, Taiwan Ministry of Education (Aim for the Top University Plan, National Yang-Ming University), National Research Program for Genomic Medicine, Taiwan (National Clinical Core, NSC-95-3112-B-001-010 and National Genotyping Core, NSC-95-3112-B-001-011) and the Genomics and Proteomics Program, Academia Sinica.

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Author notes

1. Wen-Hung Chung and Shuen-Iu Hung: These authors contributed equally to this work.

Authors and Affiliations

1. Department of Dermatology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, 199 Tunghwa North Road, Taipei, 105, Taiwan
   Wen-Hung Chung, Chien-Chun Chiou, Hsin-Chun Ho & Chih-Hsun Yang
2. Taiwan International Graduate Program, Molecular Medicine Program, Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115, Taiwan
   Wen-Hung Chung, Shuen-Iu Hung, Shih-Chi Su, Shien-Ping Huang, Chun-Yu Wei, Jer-Yuarn Wu, You-Di Liao & Yuan-Tsong Chen
3. Institute of Biochemistry and Molecular Biology, School of Life Sciences, 155 Section 2, Linong Street, Taipei, 112, Taiwan
   Wen-Hung Chung
4. Institute of Pharmacology, School of Medicine, National Yang-Ming University, 155 Section 2, Linong Street, Taipei, 112, Taiwan
   Shuen-Iu Hung & See-Wen Chin
5. Linkou Burn Center & Department of Plastic Surgery, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, 199 Tunghwa North Road, Taipei, 105, Taiwan
   Jui-Yung Yang
6. Division of Oncology and Hematology, Buddhist Tzu Chi General Hospital, 707 Section 3, Chungyang Road, Hualien, 971, Taiwan
   Sung-Chao Chu
7. Department of Dermatology, Chung Shan Hospital, 11, Lane 112, Section 4, Jenai Road, Taipei, 105, Taiwan
   Chi-Fang Lu
8. Department of Pediatrics, Duke University Medical Center, Box 103856, 595 LaSalle Street, Durham, 27710, North Carolina, USA
   Yuan-Tsong Chen

Authors

1. Wen-Hung Chung
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2. Shuen-Iu Hung
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3. Jui-Yung Yang
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4. Shih-Chi Su
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5. Shien-Ping Huang
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6. Chun-Yu Wei
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7. See-Wen Chin
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8. Chien-Chun Chiou
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9. Sung-Chao Chu
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10. Hsin-Chun Ho
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11. Chih-Hsun Yang
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12. Chi-Fang Lu
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13. Jer-Yuarn Wu
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14. You-Di Liao
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15. Yuan-Tsong Chen
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Contributions

W.-H.C. and S.-I.H. designed and conducted the experiments and data analysis and wrote the manuscript. J.-Y.Y., C.-C.C., S.-C.C., H.-C.H., C.-H.Y. and C.-F.L. cared for the involved human subjects and provided clinical samples. S.-C.S., S.-P.H., C.-Y.W. and S.-W.C. performed experiments. J.-Y.W. and Y.-D.L. helped design the expression of protein. Y.-T.C. supervised the entire project and wrote the manuscript.

Corresponding author

Correspondence toYuan-Tsong Chen.

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Competing interests

W.-H.C., S.-I.H. amd Y.-T.C. have filed a patent application to the Taiwan and US patent offices on granulysin and uses thereof.

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Chung, WH., Hung, SI., Yang, JY. et al. Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis.Nat Med 14, 1343–1350 (2008). https://doi.org/10.1038/nm.1884

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• Received: 13 June 2008
• Accepted: 30 September 2008
• Published: 23 November 2008
• Issue Date: December 2008
• DOI: https://doi.org/10.1038/nm.1884