The Mycobacterium tuberculosis protein LdtMt2 is a nonclassical transpeptidase required for virulence and resistance to amoxicillin (original) (raw)

References

  1. Wietzerbin, J. et al. Occurrence of D-alanyl-(D)-meso-diaminopimelic acid and meso-diaminopimelyl-meso-diaminopimelic acid interpeptide linkages in the peptidoglycan of Mycobacteria. Biochemistry 13, 3471–3476 (1974).
    Article CAS Google Scholar
  2. Lavollay, M. et al. The peptidoglycan of stationary-phase Mycobacterium tuberculosis predominantly contains cross-links generated by L,D-transpeptidation. J. Bacteriol. 190, 4360–4366 (2008).
    Article CAS Google Scholar
  3. Hugonnet, J.E., Tremblay, L.W., Boshoff, H.I., Barry, C.E. III & Blanchard, J.S. Meropenem-clavulanate is effective against extensively drug-resistant Mycobacterium tuberculosis. Science 323, 1215–1218 (2009).
    Article CAS Google Scholar
  4. Fauci, A.S. Multidrug-resistant and extensively drug-resistant tuberculosis: the National Institute of Allergy and Infectious Diseases Research agenda and recommendations for priority research. J. Infect. Dis. 197, 1493–1498 (2008).
    Article Google Scholar
  5. Gandhi, N.R. et al. Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa. Lancet 368, 1575–1580 (2006).
    Article Google Scholar
  6. Jindani, A., Dore, C.J. & Mitchison, D.A. Bactericidal and sterilizing activities of antituberculosis drugs during the first 14 days. Am. J. Respir. Crit. Care Med. 167, 1348–1354 (2003).
    Article Google Scholar
  7. Wayne, L.G. & Sohaskey, C.D. Nonreplicating persistence of Mycobacterium tuberculosis. Annu. Rev. Microbiol. 55, 139–163 (2001).
    Article CAS Google Scholar
  8. Betts, J.C., Lukey, P.T., Robb, L.C., McAdam, R.A. & Duncan, K. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Mol. Microbiol. 43, 717–731 (2002).
    Article CAS Google Scholar
  9. Voskuil, M.I. et al. Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program. J. Exp. Med. 198, 705–713 (2003).
    Article CAS Google Scholar
  10. Keren, I., Shah, D., Spoering, A., Kaldalu, N. & Lewis, K. Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli. J. Bacteriol. 186, 8172–8180 (2004).
    Article CAS Google Scholar
  11. Goren, M.B. & Brennan, P.J. Tuberculosis (ed. Youmans, G.P.) 63 (W. B. Saunders, Philadelphia, 1979).
  12. Vollmer, W. & Holtje, J.V. The architecture of the murein (peptidoglycan) in Gram-negative bacteria: vertical scaffold or horizontal layer(s)? J. Bacteriol. 186, 5978–5987 (2004).
    Article CAS Google Scholar
  13. Matsuhashi, M. [Biosynthesis in the bacterial cell wall] Tanpakushitsu Kakusan Koso 11, 875–886 (1966).
    CAS PubMed Google Scholar
  14. Lamichhane, G. et al. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 100, 7213–7218 (2003).
    Article CAS Google Scholar
  15. Mainardi, J.L. et al. A novel peptidoglycan cross-linking enzyme for a β-lactam–resistant transpeptidation pathway. J. Biol. Chem. 280, 38146–38152 (2005).
    Article CAS Google Scholar
  16. Lavollay, M. et al. The β-lactam-sensitive D,D-carboxypeptidase activity of Pbp4 controls the L,D and D,D transpeptidation pathways in Corynebacterium jeikeium. Mol. Microbiol. (in the press) (2009).
  17. Hugonnet, J.E. & Blanchard, J.S. Irreversible inhibition of the Mycobacterium tuberculosis β-lactamase by clavulanate. Biochemistry 46, 11998–12004 (2007).
    Article CAS Google Scholar
  18. Donald, P.R. et al. Early bactericidal activity of amoxicillin in combination with clavulanic acid in patients with sputum smear-positive pulmonary tuberculosis. Scand. J. Infect. Dis. 33, 466–469 (2001).
    Article CAS Google Scholar
  19. Nadler, J.P., Berger, J., Nord, J.A., Cofsky, R. & Saxena, M. Amoxicillin–clavulanic acid for treating drug-resistant Mycobacterium tuberculosis. Chest 99, 1025–1026 (1991).
    Article CAS Google Scholar
  20. Ghuysen, J.M. Serine β-lactamases and penicillin-binding proteins. Annu. Rev. Microbiol. 45, 37–67 (1991).
    Article CAS Google Scholar
  21. Waxman, D.J. & Strominger, J.L. Penicillin-binding proteins and the mechanism of action of β-lactam antibiotics. Annu. Rev. Biochem. 52, 825–869 (1983).
    Article CAS Google Scholar
  22. Crick, D.C. & Brennan, P.J. Biosynthesis of the arabinogalactan-peptidoglycan complex. in The Mycobacterial Cell Envelope (eds. Daffe, M. & Reyrat, J.) 25–40 (American Society for Microbiology, Washington, DC, 2008).
  23. Templin, M.F., Ursinus, A. & Holtje, J.V. A defect in cell wall recycling triggers autolysis during the stationary growth phase of Escherichia coli. EMBO J. 18, 4108–4117 (1999).
    Article CAS Google Scholar
  24. Boneca, I.G. et al. A critical role for peptidoglycan _N_-deacetylation in Listeria evasion from the host innate immune system. Proc. Natl. Acad. Sci. USA 104, 997–1002 (2007).
    Article CAS Google Scholar
  25. Lee, M.H., Pascopella, L., Jacobs, W.R. Jr. & Hatfull, G.F. Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis and bacille Calmette-Guerin. Proc. Natl. Acad. Sci. USA 88, 3111–3115 (1991).
    Article CAS Google Scholar
  26. Amrein, K.E. et al. Purification and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL. Proc. Natl. Acad. Sci. USA 92, 1048–1052 (1995).
    Article CAS Google Scholar
  27. Auger, G., van Heijenoort, J., Mengin-Lecreulx, D. & Blanot, D.A. MurG assay which utilises a synthetic analogue of lipid I. FEMS Microbiol. Lett. 219, 115–119 (2003).
    Article CAS Google Scholar
  28. Arbeloa, A. et al. Synthesis of mosaic peptidoglycan cross-bridges by hybrid peptidoglycan assembly pathways in Gram-positive bacteria. J. Biol. Chem. 279, 41546–41556 (2004).
    Article CAS Google Scholar
  29. Wiegand, I., Hilpert, K. & Hancock, R.E. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3, 163–175 (2008).
    Article CAS Google Scholar

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