The intracellular Ca2+ channel MCOLN1 is required for sarcolemma repair to prevent muscular dystrophy (original) (raw)

• Letter
• Published: 14 September 2014
• Xiaoli Zhang1,
• Qiong Gao1,
• Mohammad Ali Samie1,
• Marlene Azar1,
• Wai Lok Tsang1,
• Libing Dong1,
• Nirakar Sahoo1,
• Xinran Li1,
• Yue Zhuo1,
• Abigail G Garrity1,2,
• Xiang Wang1,
• Marc Ferrer3,
• James Dowling4,5,6,7,8,
• Li Xu9,
• Renzhi Han9 &
• …
• Haoxing Xu1,2

Nature Medicine volume 20, pages 1187–1192 (2014)Cite this article

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Abstract

The integrity of the plasma membrane is maintained through an active repair process, especially in skeletal and cardiac muscle cells, in which contraction-induced mechanical damage frequently occurs in vivo1,2. Muscular dystrophies (MDs) are a group of muscle diseases characterized by skeletal muscle wasting and weakness3,4. An important cause of these group of diseases is defective repair of sarcolemmal injuries, which normally requires Ca2+ sensor proteins5,6,7,8 and Ca2+-dependent delivery of intracellular vesicles to the sites of injury8,9. MCOLN1 (also known as TRPML1, ML1) is an endosomal and lysosomal Ca2+ channel whose human mutations cause mucolipidosis IV (ML4), a neurodegenerative disease with motor disabilities10,11. Here we report that ML1-null mice develop a primary, early-onset MD independent of neural degeneration. Although the dystrophin-glycoprotein complex and the known membrane repair proteins are expressed normally, membrane resealing was defective in ML1-null muscle fibers and also upon acute and pharmacological inhibition of ML1 channel activity or vesicular Ca2+ release. Injury facilitated the trafficking and exocytosis of vesicles by upmodulating ML1 channel activity. In the dystrophic mdx mouse model, overexpression of ML1 decreased muscle pathology. Collectively, our data have identified an intracellular Ca2+ channel that regulates membrane repair in skeletal muscle via Ca2+-dependent vesicle exocytosis.

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Acknowledgements

This work was supported by National Institutes of Health (NIH) grants (NS062792, MH096595 and AR060837 to H.X.; HL116546 and AR064241 to R.H.). We are grateful to S. Slaugenhaupt for the ML1 KO mice, L. Looger for the GCaMP3 construct, R. Edwards for the Vamp7-pHluorin construct, the Center for Live-Cell Imaging at the University of Michigan for the help on TIRF Imaging, and R. Hume and M. Akaaboune for comments on the manuscript. We appreciate the encouragement and helpful comments of other members of the Xu laboratory.

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Authors and Affiliations

1. Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA
   Xiping Cheng, Xiaoli Zhang, Qiong Gao, Mohammad Ali Samie, Marlene Azar, Wai Lok Tsang, Libing Dong, Nirakar Sahoo, Xinran Li, Yue Zhuo, Abigail G Garrity, Xiang Wang & Haoxing Xu
2. Neuroscience Graduate Program, University of Michigan, Ann Arbor, Michigan, USA
   Abigail G Garrity & Haoxing Xu
3. National Center for Advancing Translational Science, National Institutes of Health, Maryland, USA
   Marc Ferrer
4. Department of Pediatrics, University of Michigan Medical Center, Ann Arbor, Michigan, USA
   James Dowling
5. Division of Neurology, Hospital for Sick Children, Toronto, Ontario, Canada
   James Dowling
6. Program of Genetic and Genome Biology, Hospital for Sick Children, Toronto, Ontario, Canada,
   James Dowling
7. Department of Pediatrics, University of Toronto, Toronto, Ontario, Canada,
   James Dowling
8. Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
   James Dowling
9. Department of Cell and Molecular Physiology, Loyola University Chicago Health Sciences Division, Chicago, Illinois, USA
   Li Xu & Renzhi Han

Authors

1. Xiping Cheng
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2. Xiaoli Zhang
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3. Qiong Gao
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4. Mohammad Ali Samie
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5. Marlene Azar
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6. Wai Lok Tsang
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7. Libing Dong
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8. Nirakar Sahoo
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9. Xinran Li
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10. Yue Zhuo
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11. Abigail G Garrity
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12. Xiang Wang
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13. Marc Ferrer
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14. James Dowling
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15. Li Xu
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16. Renzhi Han
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17. Haoxing Xu
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Contributions

X.C. initiated the project; H.X., X.C., J.D. and R.H. designed the research; X.C., X.Z., Q.G., M.A.S., M.A., W.L.T., Y.Z. and L.D. performed the research; N.S., X.L., A.G.G., X.W., M.F. and L.X. contributed the new reagents; X.C., X.Z., Q.G., M.A.S., M.A., W.L.T., J.D., R.H. and H.X. analyzed the data; H.X. and X.C. wrote the paper with input from all the authors.

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Correspondence toXiping Cheng or Haoxing Xu.

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Cheng, X., Zhang, X., Gao, Q. et al. The intracellular Ca2+ channel MCOLN1 is required for sarcolemma repair to prevent muscular dystrophy.Nat Med 20, 1187–1192 (2014). https://doi.org/10.1038/nm.3611

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• Received: 05 March 2014
• Accepted: 19 May 2014
• Published: 14 September 2014
• Issue Date: October 2014
• DOI: https://doi.org/10.1038/nm.3611

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