Kerem, B. et al. Identification of the cystic fibrosis gene: genetic analysis. Science245, 1073–1080 (1989). ArticleCAS Google Scholar
Welsh, M.J., Ramsey, B.W., Accurso, F. & Cutting, G.R. Cystic fibrosis. in The Metabolic & Molecular Bases of Inherited Disease (eds. Scriver, C.R., Beaudet, A.L., Sly, W.S. & Valle, D.) 5121–5188 (McGraw-Hill, New York, 2001). Google Scholar
Anderson, M.P. et al. Demonstration that CFTR is a chloride channel by alteration of its anion selectivity. Science253, 202–205 (1991). ArticleCAS Google Scholar
Smith, J.J., Travis, S.M., Greenberg, E.P. & Welsh, M.J. Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface fluid. Cell85, 229–236 (1996). ArticleCAS Google Scholar
Zabner, J., Smith, J.J., Karp, P.H., Widdicombe, J.H. & Welsh, M.J. Loss of CFTR chloride channels alters salt absorption by cystic fibrosis airway epithelia in vitro. Mol. Cell2, 397–403 (1998). ArticleCAS Google Scholar
Canessa, C.M. et al. Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits. Nature367, 463–467 (1994). ArticleCAS Google Scholar
Stutts, M.J. et al. CFTR as a cAMP-dependent regulator of sodium channels. Science269, 847–850 (1995). ArticleCAS Google Scholar
Mall, M., Hipper, A., Greger, R. & Kunzelmann, K. Wild type but not F508 CFTR inhibits Na+ conductance when coexpressed in Xenopus oocytes. FEBS Lett.381, 47–52 (1996). ArticleCAS Google Scholar
Mall, M., Bleich, M., Greger, R., Schreiber, R. & Kunzelmann, K. The amiloride inhibitable Na+ conductance is reduced by CFTR in normal but not in cystic fibrosis airways. J. Clin. Invest.102, 15–21 (1998). ArticleCAS Google Scholar
Matsui, H. et al. Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease. Cell95, 1005–1015 (1998). ArticleCAS Google Scholar
Knowles, M.R. & Boucher, R.C. Mucus clearance as a primary innate defense mechanism for mammalian airways. J. Clin. Invest.109, 571–577 (2002). ArticleCAS Google Scholar
Wine, J.J. The genesis of cystic fibrosis lung disease. J. Clin. Invest.103, 309–312 (1999). ArticleCAS Google Scholar
Guggino, W.B. Cystic fibrosis and the salt controversy. Cell96, 607–610 (1999). ArticleCAS Google Scholar
Jayaraman, S., Joo, N.S., Reitz, B., Wine, J.J. & Verkman, A.S. Submucosal gland secretions in airways from cystic fibrosis patients have normal [Na+] and pH but elevated viscosity. Proc. Natl. Acad. Sci. USA98, 8119–8123 (2001). ArticleCAS Google Scholar
Verkman, A.S., Song, Y. & Thiagarajah, J.R. Role of airway surface liquid and submucosal glands in cystic fibrosis lung disease. Am. J. Physiol. Cell. Physiol.284, C2–C15 (2003). ArticleCAS Google Scholar
Heeckeren, A. et al. Excessive inflammatory response of cystic fibrosis mice to bronchopulmonary infection with Pseudomonas aeruginosa. J. Clin. Invest.100, 2810–2815 (1997). ArticleCAS Google Scholar
Snouwaert, J.N. et al. An animal model for cystic fibrosis made by gene targeting. Science257, 1083–1088 (1992). ArticleCAS Google Scholar
Grubb, B.R. & Boucher, R.C. Pathophysiology of gene-targeted mouse models for cystic fibrosis. Physiol. Rev.79, S193–S214 (1999). ArticleCAS Google Scholar
Hackett, B.P. & Gitlin, J.D. Cell-specific expression of a Clara cell secretory protein-human growth hormone gene in the bronchiolar epithelium of transgenic mice. Proc. Natl. Acad. Sci. USA89, 9079–9083 (1992). ArticleCAS Google Scholar
Ahn, Y.J. et al. Cloning and functional expression of the mouse epithelial sodium channel. Am. J. Physiol.277, F121–F129 (1999). ArticleCAS Google Scholar
Sims, D.E. & Horne, M.M. Heterogeneity of the composition and thickness of tracheal mucus in rats. Am. J. Physiol.273, L1036–L1041 (1997). ArticleCAS Google Scholar
Grubb, B.R., Jones, J.H. & Boucher, R.C. Mucociliary transport determined by in vivo microdialysis in the airways of normal and CF mice. Am. J. Physiol. Lung Cell. Mol. Physiol.286, L588–L595 (2004). ArticleCAS Google Scholar
Elias, J.A., Zhu, Z., Chupp, G. & Homer, R.J. Airway remodeling in asthma. J. Clin. Invest.104, 1001–1006 (1999). ArticleCAS Google Scholar
Boucher, R.C. Human airway ion transport. Part two. Am. J. Respir. Crit. Care Med.150, 581–593 (1994). ArticleCAS Google Scholar
Knowles, M.R. et al. Abnormal ion permeation through cystic fibrosis respiratory epithelium. Science221, 1067–1070 (1983). ArticleCAS Google Scholar
Boucher, R.C., Stutts, M.J., Knowles, M.R., Cantley, L. & Gatzy, J.T. Na+ transport in cystic fibrosis respiratory epithelia. Abnormal basal rate and response to adenylate cyclase activation. J. Clin. Invest.78, 1245–1252 (1986). ArticleCAS Google Scholar
Zuelzer, W.W. & Newton, W.A. The pathogenesis of fibrocystic disease of the pancreas; a study of 36 cases with special reference to the pulmonary lesions. Pediatrics4, 53–69 (1949). CASPubMed Google Scholar
Wanner, A., Salathe, M. & O'Riordan, T.G. Mucociliary clearance in the airways. Am. J. Respir. Crit. Care Med.154, 1868–1902 (1996). ArticleCAS Google Scholar
Breuer, R., Christensen, T.G., Lucey, E.C., Stone, P.J. & Snider, G.L. An ultrastructural morphometric analysis of elastase-treated hamster bronchi shows discharge followed by progressive accumulation of secretory granules. Am. Rev. Respir. Dis.136, 698–703 (1987). ArticleCAS Google Scholar
Takeyama, K. et al. Neutrophil-dependent goblet cell degranulation: role of membrane-bound elastase and adhesion molecules. Am. J. Physiol.275, L294–L302 (1998). CASPubMed Google Scholar
Voynow, J.A. et al. Neutrophil elastase increases MUC5AC mRNA and protein expression in respiratory epithelial cells. Am. J. Physiol.276, L835–L843 (1999). CASPubMed Google Scholar
Nakamura, H., Yoshimura, K., McElvaney, N.G. & Crystal, R.G. Neutrophil elastase in respiratory epithelial lining fluid of individuals with cystic fibrosis induces interleukin-8 gene expression in a human bronchial epithelial cell line. J. Clin. Invest.89, 1478–1484 (1992). ArticleCAS Google Scholar
Takeyama, K. et al. Epidermal growth factor system regulates mucin production in airways. Proc. Natl. Acad. Sci. USA96, 3081–3086 (1999). ArticleCAS Google Scholar
Fujii, T., Hayashi, S., Hogg, J.C., Vincent, R. & Van Eeden, S.F. Particulate matter induces cytokine expression in human bronchial epithelial cells. Am. J. Respir. Cell. Mol. Biol.25, 265–271 (2001). ArticleCAS Google Scholar
Matthews, L.M., Spector, S., Lemm, J. & Potter, J.L. Studies on pulmonary secretions. Am. Rev. Respir. Dis.88, 199–204 (1963). CASPubMed Google Scholar
Berger, M. Lung inflammation early in cystic fibrosis: bugs are indicted, but the defense is guilty. Am. J. Respir. Crit. Care Med.165, 857–858 (2002). Article Google Scholar
Tarran, R. et al. The CF salt controversy: in vivo observations and therapeutic approaches. Mol. Cell8, 149–158 (2001). ArticleCAS Google Scholar
Chen, J., Knowles, H.J., Hebert, J.L. & Hackett, B.P. Mutation of the mouse hepatocyte nuclear factor/forkhead homologue 4 gene results in an absence of cilia and random left-right asymmetry. J. Clin. Invest.102, 1077–1082 (1998). ArticleCAS Google Scholar
Brody, S.L., Yan, X.H., Wuerffel, M.K., Song, S.K. & Shapiro, S.D. Ciliogenesis and left-right axis defects in forkhead factor HFH-4-null mice. Am. J. Respir. Cell. Mol. Biol.23, 45–51 (2000). ArticleCAS Google Scholar
Kobayashi, Y. et al. Hydrocephalus, situs inversus, chronic sinusitis, and male infertility in DNA polymerase lambda-deficient mice: possible implication for the pathogenesis of immotile cilia syndrome. Mol. Cell. Biol.22, 2769–2776 (2002). ArticleCAS Google Scholar
Ibanez-Tallon, I., Gorokhova, S. & Heintz, N. Loss of function of axonemal dynein Mdnah5 causes primary ciliary dyskinesia and hydrocephalus. Hum. Mol. Genet.11, 715–721 (2002). ArticleCAS Google Scholar
Puchelle, E., de Bentzmann, S. & Zahm, J.M. Physical and functional properties of airway secretions in cystic fibrosis - therapeutic approaches. Respiration62 (suppl. 1), 2–12 (1995). ArticleCAS Google Scholar
Kim, C.S., Rodriguez, C.R., Eldridge, M.A. & Sackner, M.A. Criteria for mucus transport in the airways by two-phase gas-liquid flow mechanism. J. Appl. Physiol.60, 901–907 (1986). ArticleCAS Google Scholar
Masilamani, S., Kim, G.H., Mitchell, C., Wade, J.B. & Knepper, M.A. Aldosterone-mediated regulation of ENaC α, β, and γ subunit proteins in rat kidney. J. Clin. Invest.104, R19–R23 (1999). ArticleCAS Google Scholar
Loffing, J. et al. Differential subcellular localization of ENaC subunits in mouse kidney in response to high- and low-Na diets. Am. J. Physiol. Renal Physiol.279, F252–F258 (2000). ArticleCAS Google Scholar
Fyfe, G.K. & Canessa, C.M. Subunit composition determines the single channel kinetics of the epithelial sodium channel. J. Gen. Physiol.112, 423–432 (1998). ArticleCAS Google Scholar
Baker, E.H. et al. Association of hypertension with T594M mutation in beta subunit of epithelial sodium channels in black people resident in London. Lancet351, 1388–1392 (1998). ArticleCAS Google Scholar
Ostrowski, L.E., Hutchins, J.R., Zakel, K. & O'Neal, W.K. Targeting expression of a transgene to the airway surface epithelium using a ciliated cell-specific promoter. Mol. Ther.8, 637–645 (2003). ArticleCAS Google Scholar
Grubb, B.R., Pace, A.J., Lee, E., Koller, B.H. & Boucher, R.C. Alterations in airway ion transport in NKCC1-deficient mice. Am. J. Physiol. Cell. Physiol.281, C615–C623 (2001). ArticleCAS Google Scholar
Grunig, G. et al. Requirement for IL-13 independently of IL-4 in experimental asthma. Science282, 2261–2263 (1998). ArticleCAS Google Scholar