Targeting gene-modified hematopoietic cells to the central nervous system: Use of green fluorescent protein uncovers microglial engraftment (original) (raw)

References

  1. Kreutzberg, G.W. Microglia: a sensor for pathological events in the CNS. Trends Neurosci. 19, 312–318 (1996).
    Article CAS Google Scholar
  2. Eglitis, M.A. & Mezey, É. Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice. Proc. Natl. Acad. Sci. USA 94, 4080–4085 (1997).
    Article CAS Google Scholar
  3. Brazelton, T.R., Rossi, F.M.V., Keshet, G.I. & Blau, H.M. From marrow to brain: expression of neuronal phenotypes in adult mice. Science 290, 1775–1779 (2000).
    Article CAS Google Scholar
  4. Mezey, É, Chandross, K.J., Harta, G., Maki, R.A. & McKercher, S.R. Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow. Science 290, 1779–1782 (2000).
    Article CAS Google Scholar
  5. Persons, D.A. et al. Retroviral-mediated transfer of the green fluorescent protein gene into murine hematopoietic cells facilitates scoring and selection of transduced progenitors in vitro and identification of genetically modified cells in vivo. Blood 90, 1777–1786 (1997).
    CAS Google Scholar
  6. Pawliuk, R., Eaves, C. & Humphries, R. Sustained high-level reconstitution of the hematopoietic system by preselected hematopoietic cells expressing a transduced cell-surface antigen. Hum. Gene Ther. 8, 1595–1604 (1997).
    Article CAS Google Scholar
  7. Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y. Green mice as a source of ubiquitous green cells. FEBS Lett. 407, 313–319 (1997).
    Article CAS Google Scholar
  8. Imai, Y. et al. A novel gene Iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage. Biochem. Biophys. Res. Commun. 224, 855–862 (1996).
    Article CAS Google Scholar
  9. Carson, M.J., Reilly, C.R., Sutcliffe, J.G. & Lo, D. Mature microglia resemble immature antigen-presenting cells Glia 22, 72–85 (1998).
    Article CAS Google Scholar
  10. Hollerbach, E.H., Haas, C.A., Hildebrandt, H., Frotscher, M. & Naumann, T. Region-specific activation of microglial cells in the rat septal complex following fimbria-fornix transection. J. Comp. Neurol. 390, 481–496 (1998).
    Article CAS Google Scholar
  11. Kennedy, D.W. & Abkowitz, J.L. Mature monocytic cells enter tissues and engraft. Proc. Natl. Acad. Sci. USA 95, 14944–14949 (1998).
    Article CAS Google Scholar
  12. Lagasse, E. et al. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nature Med. 6, 1229–1234 (2000).
    Article CAS Google Scholar
  13. Orlic, D. et al. Bone marrow cells regenerate infarcted myocardium. Nature 410, 701–705 (2001).
    Article CAS Google Scholar
  14. Krall, W.J. et al. Cells expressing human glucocerebrosidase from a retroviral vector repopulate macrophages and central nervous system microglia after murine bone marrow transplantation. Blood 83, 2737–2748 (1994).
    CAS PubMed Google Scholar
  15. Hickey, W.F. & Kimura, H. Perivascular microglial cells of the CNS are bone marrow-derived and present antigen in vivo. Science 239, 290–292 (1988).
    Article CAS Google Scholar
  16. Hickey, W.F., Vass, K. & Lassmann, H. Bone marrow-derived elements in the central nervous system: an immunohistochemical and ultrastructural survey of rat chimeras. J. Neuropathol. Exp. Neurol. 51, 246–256 (1992).
    Article CAS Google Scholar
  17. Goldman, S. & Roy, N. Human neural progenitor cells: better blue than green? Nature Med. 6, 483–484 (2000).
    Article CAS Google Scholar
  18. Kennedy, D.W. & Abkowitz, J.L. Kinetics of central nervous system microglial and macrophage engraftment: Analysis using a transgenic bone marrow transplantation model. Blood 90, 986–993 (1997).
    CAS PubMed Google Scholar
  19. Schlüter, D. et al. Regulation of microglia by CD4+ and CD8+ T cells: selective analysis in CD45-congenic normal and Toxoplasma gondii -infected bone marrow chimeras. Brain Pathol. 11, 44–55 (2001).
    Article Google Scholar
  20. Lawson, L.J., Perry, V.H. & Gordon, S. Turnover of resident microglia in the normal adult mouse brain. Neuroscience 48, 405–415 (1992).
    Article CAS Google Scholar
  21. Burt, R.K. et al. Gene-marked autologous hematopoietic stem cell transplantation of autoimmune disease. J. Clin. Immunol. 20, 1–9 (2000).
    Article CAS Google Scholar
  22. Wu, Y.-P. et al. Distribution and characterization of GFP+ donor hematogenous cells in twitcher mice after bone marrow transplantation. Am. J. Pathol. 156, 1849–1854 (2000).
    Article CAS Google Scholar
  23. Wada, R., Tifft, C.J. & Proia, R.L. Microglial activation precedes acute neurodegeneration in Sandhoff disease and is suppressed by bone marrow transplantation. Proc. Natl. Acad. Sci. USA 97, 10954–10959 (2000).
    Article CAS Google Scholar
  24. Flügel, A. et al. Neuronal MCP-1 expression in response to remote nerve injury. J. Cereb. Blood Flow Metab. 21, 60–76 (2001).
    Article Google Scholar
  25. Hara, H., Huang, P.L., Panahian, N., Fishman, M.C. & Moskowitz, M.A. Reduced brain edema and infraction in mice lacking the neuronal isoform of nitric oxide synthase after transient MCA occlusion. J. Cereb. Blood Flow Metab. 16, 605–611 (1996).
    Article CAS Google Scholar

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