Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells (original) (raw)
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07 January 2010
In the version of this article initially published, two micrographs in Figure 2c, corresponding to the conditions BSO(–) Lineage– and BSO(+) Lineage–, were incorrect. These micrographs have been replaced with the correct micrographs in the HTML and PDF versions of the article.
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Acknowledgements
We thank P.J. McKinnon for providing Atm+/− mice and H. Saya for discussion, H. Ichijo and K. Takeda for providing Map3k5 cDNA, T. Kitamura for providing the retrovirus vector pMY-IRES-EGFP and A. Ono and K. Murakami for technical support. K.I. was supported by a grant-in-aid for Young Scientists from the Ministry of Education, Science, Sports, and Culture, Japan. A.H. was supported by a grant-in-aid for the Stem Cell Research from the Ministry of Education, Science, Sports, and Culture, Japan. T.S. was supported by a grant-in-aid for Specially Promoted Research from Ministry of Education, Science, Sports, and Culture, Japan.
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- Keisuke Ito and Atsushi Hirao: These authors contributed equally to this work.
Authors and Affiliations
- Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan
Keisuke Ito, Atsushi Hirao, Fumio Arai, Keiyo Takubo, Sahoko Matsuoka, Kana Miyamoto, Masako Ohmura, Kentaro Hosokawa & Toshio Suda - Division of Hematology, Department of Internal Medicine, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan
Keisuke Ito, Sahoko Matsuoka & Yasuo Ikeda - Division of Molecular Genetics, Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan
Atsushi Hirao, Masako Ohmura & Kazuhito Naka - Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan
Atsushi Hirao
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Supplementary information
Supplementary Fig. 1
Effect of increased ROS on HSCs and progenitor cells. (PDF 4273 kb)
Supplementary Fig. 2
Atm −/− HSCs are highly sensitive to ROS elevation in terms of p38 MAPK activation. (PDF 1639 kb)
Supplementary Fig. 3
Treatment with a p38 MAPK inhibitor rescues defective HSC function in Atm −/− mice. (PDF 5444 kb)
Supplementary Fig. 4
Elevation of ROS level and _p16_Ink4a/_p19_Arf expression in KSL cells during aging and serial transplantation. (PDF 3302 kb)
Supplementary Fig. 5
In vitro treatment with a p38 MAPK inhibitor restores BSO-induced defective repopulating capacity of HSCs. (PDF 2665 kb)
Supplementary Table 1
List of RT-PCR primers used in this study. (PDF 5052 kb)
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Ito, K., Hirao, A., Arai, F. et al. Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells.Nat Med 12, 446–451 (2006). https://doi.org/10.1038/nm1388
- Received: 10 November 2005
- Accepted: 27 February 2006
- Published: 26 March 2006
- Issue Date: 01 April 2006
- DOI: https://doi.org/10.1038/nm1388