Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells (original) (raw)

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In the version of this article initially published, two micrographs in Figure 2c, corresponding to the conditions BSO(–) Lineage– and BSO(+) Lineage–, were incorrect. These micrographs have been replaced with the correct micrographs in the HTML and PDF versions of the article.

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Acknowledgements

We thank P.J. McKinnon for providing Atm+/− mice and H. Saya for discussion, H. Ichijo and K. Takeda for providing Map3k5 cDNA, T. Kitamura for providing the retrovirus vector pMY-IRES-EGFP and A. Ono and K. Murakami for technical support. K.I. was supported by a grant-in-aid for Young Scientists from the Ministry of Education, Science, Sports, and Culture, Japan. A.H. was supported by a grant-in-aid for the Stem Cell Research from the Ministry of Education, Science, Sports, and Culture, Japan. T.S. was supported by a grant-in-aid for Specially Promoted Research from Ministry of Education, Science, Sports, and Culture, Japan.

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Author notes

  1. Keisuke Ito and Atsushi Hirao: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan
    Keisuke Ito, Atsushi Hirao, Fumio Arai, Keiyo Takubo, Sahoko Matsuoka, Kana Miyamoto, Masako Ohmura, Kentaro Hosokawa & Toshio Suda
  2. Division of Hematology, Department of Internal Medicine, Keio University School of Medicine, 35 Shinano-machi, Shinjuku-ku, Tokyo, 160-8582, Japan
    Keisuke Ito, Sahoko Matsuoka & Yasuo Ikeda
  3. Division of Molecular Genetics, Department of Molecular Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan
    Atsushi Hirao, Masako Ohmura & Kazuhito Naka
  4. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan
    Atsushi Hirao

Authors

  1. Keisuke Ito
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  2. Atsushi Hirao
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  3. Fumio Arai
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  4. Keiyo Takubo
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  5. Sahoko Matsuoka
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  6. Kana Miyamoto
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  7. Masako Ohmura
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  8. Kazuhito Naka
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  9. Kentaro Hosokawa
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  10. Yasuo Ikeda
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  11. Toshio Suda
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Corresponding authors

Correspondence toAtsushi Hirao or Toshio Suda.

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Supplementary information

Supplementary Fig. 1

Effect of increased ROS on HSCs and progenitor cells. (PDF 4273 kb)

Supplementary Fig. 2

Atm −/− HSCs are highly sensitive to ROS elevation in terms of p38 MAPK activation. (PDF 1639 kb)

Supplementary Fig. 3

Treatment with a p38 MAPK inhibitor rescues defective HSC function in Atm −/− mice. (PDF 5444 kb)

Supplementary Fig. 4

Elevation of ROS level and _p16_Ink4a/_p19_Arf expression in KSL cells during aging and serial transplantation. (PDF 3302 kb)

Supplementary Fig. 5

In vitro treatment with a p38 MAPK inhibitor restores BSO-induced defective repopulating capacity of HSCs. (PDF 2665 kb)

Supplementary Table 1

List of RT-PCR primers used in this study. (PDF 5052 kb)

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Ito, K., Hirao, A., Arai, F. et al. Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells.Nat Med 12, 446–451 (2006). https://doi.org/10.1038/nm1388

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