In vivo wide-area cellular imaging by side-view endomicroscopy (original) (raw)

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Acknowledgements

This work was supported by the Wellman Center for Photomedicine, Human Frontier Science Program (cross-disciplinary fellowship 2006), Tosteson Fellowship, National Research Foundation of Korea (R31-2008-000-10071-0) and the US National Institutes of Health (R21AI081010, RC1DK086242, RC2DK088661, U54CA143837, U01CA084301, R01CA85140, P01CA08124, R01CA96915 and R01CA126642).

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Authors and Affiliations

  1. Department of Dermatology, Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA
    Pilhan Kim & Seok H Yun
  2. Department of Radiation Oncology, Edwin L. Steele Lab for Tumor Biology, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA
    Euiheon Chung, Hiroshi Yamashita, Dai Fukumura & Rakesh K Jain
  3. Division of Gastroenterology, Tufts Medical Center, Boston, Massachusetts, USA
    Kenneth E Hung
  4. Department of Pathology, Center for the Study of Inflammatory Bowel Disease, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA
    Atsushi Mizoguchi
  5. Department of Genetics, Brigham and Women's Hospital, Boston, Massachusetts, USA
    Raju Kucherlapati
  6. Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Korea.,
    Seok H Yun
  7. The Harvard–Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, Massachusetts, USA
    Seok H Yun

Authors

  1. Pilhan Kim
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  2. Euiheon Chung
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  3. Hiroshi Yamashita
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  4. Kenneth E Hung
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  5. Atsushi Mizoguchi
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  6. Raju Kucherlapati
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  7. Dai Fukumura
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  8. Rakesh K Jain
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  9. Seok H Yun
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Contributions

P.K. developed endomicroscopy, performed experiments and wrote the manuscript. E.C. and H.Y. performed the conditional-knockout procedure and helped with angiogenesis imaging. K.E.H. developed the conditional-knockout procedure. A.M. designed the colitis model. R.K., D.F. and R.K.J. designed the tumor model and angiogenesis study. S.H.Y. directed the overall project, developed endomicroscopy and wrote the manuscript with input from other authors.

Corresponding author

Correspondence toSeok H Yun.

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Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–9 (PDF 1601 kb)

Supplementary Video 1

Virtual cellular colonoscopy visualizing the microvasculature of normal colonic mucosa. (MOV 4039 kb)

Supplementary Video 2

Real-time endoscopic view of the blood vessels in the normal colon. (MOV 2730 kb)

Supplementary Video 3

Fly-through movie showing the blood vessels of esophageal mucosa. (MOV 2246 kb)

Supplementary Video 4

Time-lapse movie showing the interaction of dendritic cells (green) and Ovalbumin (OVA) allergen (red) at 12 h after OVA challenge. (MOV 735 kb)

Supplementary Video 5

Real-time movie showing the angiogenic blood vessels near the tumor at week 16 after conditional Apc knockout. (MOV 1912 kb)

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Kim, P., Chung, E., Yamashita, H. et al. In vivo wide-area cellular imaging by side-view endomicroscopy.Nat Methods 7, 303–305 (2010). https://doi.org/10.1038/nmeth.1440

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