Flybow: genetic multicolor cell labeling for neural circuit analysis in Drosophila melanogaster (original) (raw)

References

  1. Brand, A.H. & Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401–415 (1993).
    CAS PubMed Google Scholar
  2. Golic, K.G. & Lindquist, S. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59, 499–509 (1989).
    Article CAS PubMed Google Scholar
  3. Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451–461 (1999).
    Article CAS PubMed Google Scholar
  4. Pfeiffer, B.D. et al. Tools for neuroanatomy and neurogenetics in Drosophila. Proc. Natl. Acad. Sci. USA 105, 9715–9720 (2008).
    Article CAS PubMed PubMed Central Google Scholar
  5. Luan, H., Peabody, N.C., Vinson, C.R. & White, B.H. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Neuron 52, 425–436 (2006).
    Article CAS PubMed PubMed Central Google Scholar
  6. Lai, S.L. & Lee, T. Genetic mosaic with dual binary transcriptional systems in Drosophila. Nat. Neurosci. 9, 703–709 (2006).
    Article CAS PubMed Google Scholar
  7. Yagi, R., Mayer, F. & Basler, K. Refined LexA transactivators and their use in combination with the Drosophila Gal4 system. Proc. Natl. Acad. Sci. USA 107, 16166–16171 (2010).
    Article CAS PubMed PubMed Central Google Scholar
  8. Potter, C.J., Tasic, B., Russler, E.V., Liang, L. & Luo, L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell 141, 536–548 (2010).
    Article CAS PubMed PubMed Central Google Scholar
  9. Struhl, G. & Basler, K. Organizing activity of wingless protein in Drosophila. Cell 72, 527–540 (1993).
    Article CAS PubMed Google Scholar
  10. Ito, K., Awano, W., Suzuki, K., Hiromi, Y. & Yamamoto, D. The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells. Development 124, 761–771 (1997).
    CAS PubMed Google Scholar
  11. Wong, A.M., Wang, J.W. & Axel, R. Spatial representation of the glomerular map in the Drosophila protocerebrum. Cell 109, 229–241 (2002).
    Article CAS PubMed Google Scholar
  12. Xu, T. & Rubin, G.M. Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 117, 1223–1237 (1993).
    CAS PubMed Google Scholar
  13. Livet, J. et al. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450, 56–62 (2007).
    Article CAS PubMed Google Scholar
  14. Fischbach, K.F. & Dittrich, A.P.M. The optic lobe of Drosophila melanogaster. I. A Golgi analysis of wild-type structure. Cell Tissue Res. 258, 441–475 (1989).
    Article Google Scholar
  15. Hadjieconomou, D., Timofeev, K. & Salecker, I. A step-by-step guide to visual circuit assembly in Drosophila. Curr. Opin. Neurobiol. doi:10.1016/j.conb.2010.07.012 (2010).
  16. Siegal, M.L. & Hartl, D.L. Transgene coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila. Genetics 144, 715–726 (1996).
    CAS PubMed PubMed Central Google Scholar
  17. Heidmann, D. & Lehner, C.F. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity regulation. Dev. Genes Evol. 211, 458–465 (2001).
    Article CAS PubMed Google Scholar
  18. Voziyanov, Y., Konieczka, J.H., Stewart, A.F. & Jayaram, M. Stepwise manipulation of DNA specificity in Flp recombinase: progressively adapting Flp to individual and combinatorial mutations in its target site. J. Mol. Biol. 326, 65–76 (2003).
    Article CAS PubMed Google Scholar
  19. Branda, C.S. & Dymecki, S.M. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev. Cell 6, 7–28 (2004).
    Article CAS PubMed Google Scholar
  20. Dietzl, G. et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448, 151–156 (2007).
    Article CAS PubMed Google Scholar
  21. Groth, A.C., Fish, M., Nusse, R. & Calos, M.P. Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 166, 1775–1782 (2004).
    Article CAS PubMed PubMed Central Google Scholar
  22. Shaner, N.C., Steinbach, P.A. & Tsien, R.Y. A guide to choosing fluorescent proteins. Nat. Methods 2, 905–909 (2005).
    Article CAS PubMed Google Scholar
  23. Morante, J. & Desplan, C. The color-vision circuit in the medulla of Drosophila. Curr. Biol. 18, 553–565 (2008).
    Article CAS PubMed PubMed Central Google Scholar
  24. Brewster, R. & Bodmer, R. Origin and specification of type II sensory neurons in Drosophila. Development 121, 2923–2936 (1995).
    CAS PubMed Google Scholar
  25. Pearson, B.J. & Doe, C.Q. Regulation of neuroblast competence in Drosophila. Nature 425, 624–628 (2003).
    Article CAS PubMed Google Scholar
  26. Senti, K.A. et al. Flamingo regulates R8 axon-axon and axon-target interactions in the Drosophila visual system. Curr. Biol. 13, 828–832 (2003).
    Article CAS PubMed Google Scholar
  27. Erclik, T., Hartenstein, V., McInnes, R.R. & Lipshitz, H.D. Eye evolution at high resolution: the neuron as a unit of homology. Dev. Biol. 332, 70–79 (2009).
    Article CAS PubMed Google Scholar
  28. Nern, A., Zhu, Y. & Zipursky, S.L. Local N-cadherin interactions mediate distinct steps in the targeting of lamina neurons. Neuron 58, 34–41 (2008).
    Article CAS PubMed PubMed Central Google Scholar
  29. Goedhart, J. et al. Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Nat. Methods 7, 137–139 (2010).
    Article CAS PubMed Google Scholar
  30. Millard, S.S., Flanagan, J.J., Pappu, K.S., Wu, W. & Zipursky, S.L. Dscam2 mediates axonal tiling in the Drosophila visual system. Nature 447, 720–724 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  31. Liaw, C.W., Zamoyska, R. & Parnes, J.R. Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene. J. Immunol. 137, 1037–1043 (1986).
    CAS PubMed Google Scholar
  32. Zamoyska, R., Vollmer, A.C., Sizer, K.C., Liaw, C.W. & Parnes, J.R. Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA. Cell 43, 153–163 (1985).
    Article CAS PubMed Google Scholar
  33. Zacharias, D.A., Violin, J.D., Newton, A.C. & Tsien, R.Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913–916 (2002).
    Article CAS PubMed Google Scholar
  34. Griesbeck, O., Baird, G.S., Campbell, R.E., Zacharias, D.A. & Tsien, R.Y. Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J. Biol. Chem. 276, 29188–29194 (2001).
    Article CAS PubMed Google Scholar
  35. Shaner, N.C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–1572 (2004).
    Article CAS PubMed Google Scholar
  36. Rizzo, M.A., Springer, G.H., Granada, B. & Piston, D.W. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22, 445–449 (2004).
    Article CAS PubMed Google Scholar
  37. Newsome, T.P., Asling, B. & Dickson, B.J. Analysis of Drosophila photoreceptor axon guidance in eye-specific mosaics. Development 127, 851–860 (2000).
    CAS PubMed Google Scholar
  38. Bischof, J., Maeda, R.K., Hediger, M., Karch, F. & Basler, K. An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc. Natl. Acad. Sci. USA 104, 3312–3317 (2007).
    Article CAS PubMed PubMed Central Google Scholar
  39. Lin, D.M. & Goodman, C.S. Ectopic and increased expression of Fasciclin II alters motoneuron growth cone guidance. Neuron 13, 507–523 (1994).
    Article CAS PubMed Google Scholar
  40. Landgraf, M., Sanchez-Soriano, N., Technau, G.M., Urban, J. & Prokop, A. Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites. Dev. Biol. 260, 207–225 (2003).
    Article CAS PubMed Google Scholar
  41. Sepp, K.J. & Auld, V.J. Reciprocal interactions between neurons and glia are required for Drosophila peripheral nervous system development. J. Neurosci. 23, 8221–8230 (2003).
    Article CAS PubMed PubMed Central Google Scholar
  42. Tabata, T., Schwartz, C., Gustavson, E., Ali, Z. & Kornberg, T.B. Creating a Drosophila wing de novo, the role of engrailed, and the compartment border hypothesis. Development 121, 3359–3369 (1995).
    CAS PubMed Google Scholar

Download references