Improved Mos1-mediated transgenesis in C. elegans (original) (raw)
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- Published: 30 January 2012
Nature Methods volume 9, pages 117–118 (2012)Cite this article
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To the Editor:
The ability to add or delete genes to the genome of genetic model organisms is essential. Previously, we had developed methods based on the Mos1 transposon1 to make targeted transgene insertions (_Mos1_-mediated single-copy transgene insertions; MosSCI2) and targeted deletions (_Mos1_-mediated deletions; MosDEL3) in Caenorhabditis elegans, the latter published in Nature Methods. Here we present new reagents that improve the efficiency, facilitate the selection for transgenic strains and expand the set of MosSCI insertion sites (Supplementary Table 1).
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Figure 1: Improvements to _Mos1_-based genome manipulation.
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Acknowledgements
C.F.-J. was funded by postdoctoral stipends from the Lundbeck foundation and the Carlsberg foundation. M.A. was supported by a Helen Hay Whitney Postdoctoral Fellowship and by the US National Institutes of Health (K99MH082109). This research was funded by US National Institutes of Health (1R01GM095817) and Howard Hughes Medical Institute.
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- Michael Ailion
Present address: Present address: Department of Biochemistry, University of Washington, Seattle, Washington, USA.,
Authors and Affiliations
- Department of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA
Christian Frøkjær-Jensen, M Wayne Davis, Michael Ailion & Erik M Jorgensen - Department of Biomedical Sciences and Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark
Christian Frøkjær-Jensen
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Correspondence toErik M Jorgensen.
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E.M.J. is an author of a patent covering techniques described in this paper (US patent 7,196,244 and European patent pending).
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Frøkjær-Jensen, C., Davis, M., Ailion, M. et al. Improved _Mos1_-mediated transgenesis in C. elegans.Nat Methods 9, 117–118 (2012). https://doi.org/10.1038/nmeth.1865
- Published: 30 January 2012
- Issue Date: February 2012
- DOI: https://doi.org/10.1038/nmeth.1865