Improved Mos1-mediated transgenesis in C. elegans (original) (raw)

Nature Methods volume 9, pages 117–118 (2012)Cite this article

Subjects

To the Editor:

The ability to add or delete genes to the genome of genetic model organisms is essential. Previously, we had developed methods based on the Mos1 transposon1 to make targeted transgene insertions (_Mos1_-mediated single-copy transgene insertions; MosSCI2) and targeted deletions (_Mos1_-mediated deletions; MosDEL3) in Caenorhabditis elegans, the latter published in Nature Methods. Here we present new reagents that improve the efficiency, facilitate the selection for transgenic strains and expand the set of MosSCI insertion sites (Supplementary Table 1).

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Subscribe to this journal

Receive 12 print issues and online access

$259.00 per year

only $21.58 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Figure 1: Improvements to _Mos1_-based genome manipulation.

References

  1. Robert, V. & Bessereau, J.L. EMBO J. 26, 170–183 (2007).
    Article CAS Google Scholar
  2. Frøkjær-Jensen, C. et al. Nat. Genet. 40, 1375–1383 (2008).
    Article Google Scholar
  3. Frøkjær-Jensen, C. et al. Nat. Methods 7, 451–453 (2010).
    Article Google Scholar
  4. Seidel, H.S. et al. PLoS Biol. 9, e1001115 (2011).
    Article CAS Google Scholar
  5. Giordano-Santini, R. et al. Nat. Methods 7, 721–723 (2010).
    Article CAS Google Scholar
  6. Semple, J.I., Garcia-Verdugo, R. & Lehner, B. Nat. Methods 7, 725–727 (2010).
    Article CAS Google Scholar

Download references

Acknowledgements

C.F.-J. was funded by postdoctoral stipends from the Lundbeck foundation and the Carlsberg foundation. M.A. was supported by a Helen Hay Whitney Postdoctoral Fellowship and by the US National Institutes of Health (K99MH082109). This research was funded by US National Institutes of Health (1R01GM095817) and Howard Hughes Medical Institute.

Author information

Author notes

  1. Michael Ailion
    Present address: Present address: Department of Biochemistry, University of Washington, Seattle, Washington, USA.,

Authors and Affiliations

  1. Department of Biology, Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA
    Christian Frøkjær-Jensen, M Wayne Davis, Michael Ailion & Erik M Jorgensen
  2. Department of Biomedical Sciences and Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark
    Christian Frøkjær-Jensen

Authors

  1. Christian Frøkjær-Jensen
    You can also search for this author inPubMed Google Scholar
  2. M Wayne Davis
    You can also search for this author inPubMed Google Scholar
  3. Michael Ailion
    You can also search for this author inPubMed Google Scholar
  4. Erik M Jorgensen
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toErik M Jorgensen.

Ethics declarations

Competing interests

E.M.J. is an author of a patent covering techniques described in this paper (US patent 7,196,244 and European patent pending).

Supplementary information

Rights and permissions

About this article

Cite this article

Frøkjær-Jensen, C., Davis, M., Ailion, M. et al. Improved _Mos1_-mediated transgenesis in C. elegans.Nat Methods 9, 117–118 (2012). https://doi.org/10.1038/nmeth.1865

Download citation

This article is cited by