Fluorogenic probes for live-cell imaging of the cytoskeleton (original) (raw)

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Acknowledgements

The authors are grateful to J.E. Bear (University of North Carolina at Chapel Hill), P. Gönczy (École Polytechnique Fédérale de Lausanne (EPFL)), R. Jahn (University of Göttingen), D. Abankwa (Åbo Akademi University), U. Ruegg (University of Geneva) and A. Seitz (EPFL) for sharing reagents and for technical assistance. K.J. acknowledges support from the Swiss National Science Foundation and the National Centre of Competence of Research (NCCR) Chemical Biology. Support from the Körber foundation was received through the European Science Prize to S.W.H. D.W.G. acknowledges support from the European Community's Seventh Framework Programme FP7/2007-2013 under grant agreements nos. 241548 (MitoSys) and 258068 (Systems Microscopy) and from an European Research Council (ERC) Starting Grant (agreement no. 281198).

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Author notes

  1. Gražvydas Lukinavičius and Luc Reymond: These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
    Gražvydas Lukinavičius, Luc Reymond, Anastasiya Masharina & Kai Johnsson
  2. National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland
    Luc Reymond & Kai Johnsson
  3. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
    Elisa D'Este, Fabian Göttfert, Haisen Ta & Stefan W Hell
  4. Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany
    Angelika Güther & Hans-Dieter Arndt
  5. Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
    Mathias Fournier
  6. Max Planck Institute of Molecular Physiology, Dortmund, Germany
    Stefano Rizzo & Herbert Waldmann
  7. Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria
    Claudia Blaukopf, Christoph Sommer & Daniel W Gerlich

Authors

  1. Gražvydas Lukinavičius
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  2. Luc Reymond
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  3. Elisa D'Este
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  4. Anastasiya Masharina
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  5. Fabian Göttfert
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  6. Haisen Ta
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  7. Angelika Güther
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  8. Mathias Fournier
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  9. Stefano Rizzo
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  10. Herbert Waldmann
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  11. Claudia Blaukopf
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  12. Christoph Sommer
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  13. Daniel W Gerlich
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  14. Hans-Dieter Arndt
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  15. Stefan W Hell
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  16. Kai Johnsson
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Contributions

G.L., L.R. and K.J. devised this study. All authors except S.R. and A.G. contributed to manuscript writing. G.L., E.D., A.M., C.B., C.S. and D.W.G. characterized the probes. L.R., A.G. and S.R. performed synthesis of probes, supervised by K.J., H.W. and H.-D.A.; G.L., E.D., F.G. and H.T. performed STED microscopy, guided by S.W.H.; G.L. and M.F. performed SIM microscopy.

Corresponding authors

Correspondence toHans-Dieter Arndt, Stefan W Hell or Kai Johnsson.

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Competing interests

G.L. and K.J. have filed a patent application on SiR derivatives (PCT/EP2011/064750). S.R., H.-D.A. and H.W. have filed a patent application on the use of jasplakinolide derivatives (PCT/EP2012/073002).

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Lukinavičius, G., Reymond, L., D'Este, E. et al. Fluorogenic probes for live-cell imaging of the cytoskeleton.Nat Methods 11, 731–733 (2014). https://doi.org/10.1038/nmeth.2972

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