Genome editing with modularly assembled zinc-finger nucleases (original) (raw)

Nature Methods volume 7, page 91 (2010)Cite this article

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To the Editor:

In a Correspondence in Nature Methods, some members of the Zinc Finger Consortium reported discouragingly high failure rates for the modular assembly of zinc-finger DNA-binding proteins and concluded that more time-consuming and labor-intensive selection-based methods were “the only publicly available alternatives for academic researchers interested in using ZFN technology”1. Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing reprogrammable zinc-finger DNA-binding units to the FokI nuclease domain, which efficiently induce, site-specific mutations in higher eukaryotic cells, and thus hold great promise in many fields. Three methods have been developed to make ZFNs: a proprietary method used by Sangamo Biosciences, the modular-assembly method via standard recombinant DNA technology2 and cell-based selection methods3 (Supplementary Fig. 1). Each of these methods has pros and cons, and we wish to inform potential ZFN users of them.

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Acknowledgements

J.-S.K. is supported in part by the Korean Science and Engineering Foundation (R17-2007-019-01001-0). D.C. is supported in part by US National Institutes of Health (R01 GM078571).

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  1. Department of Chemistry, Seoul National University, Seoul, South Korea
    Jin-Soo Kim & Hyung Joo Lee
  2. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah, USA
    Dana Carroll

Authors

  1. Jin-Soo Kim
  2. Hyung Joo Lee
  3. Dana Carroll

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Correspondence toJin-Soo Kim or Dana Carroll.

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The authors declare no competing financial interests.

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Kim, JS., Lee, H. & Carroll, D. Genome editing with modularly assembled zinc-finger nucleases.Nat Methods 7, 91 (2010). https://doi.org/10.1038/nmeth0210-91a

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