Genetic incorporation of unnatural amino acids into proteins in mammalian cells (original) (raw)

References

  1. Monahan, S.L., Lester, H.A. & Dougherty, D.A. Site-specific incorporation of unnatural amino acids into receptors expressed in mammalian cells. Chem. Biol. 10, 573–580 (2003).
    Article CAS Google Scholar
  2. Sakamoto, K. et al. Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells. Nucleic Acids Res. 30, 4692–4699 (2002).
    Article CAS Google Scholar
  3. Zhang, Z. et al. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. Proc. Natl. Acad. Sci. USA 101, 8882–8887 (2004).
    Article CAS Google Scholar
  4. Zhang, D., Vaidehi, N., Goddard, W.A., III, Danzer, J.F. & Debe, D. Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine. Proc. Natl. Acad. Sci. USA 99, 6579–6584 (2002).
    Article CAS Google Scholar
  5. Turner, J.M., Graziano, J., Spraggon, G. & Schultz, P.G. Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase. J. Am. Chem. Soc. 127, 14976–14977 (2005).
    Article CAS Google Scholar
  6. Turner, J.M., Graziano, J., Spraggon, G. & Schultz, P.G. Structural plasticity of an aminoacyl-tRNA synthetase active site. Proc. Natl. Acad. Sci. USA 103, 6483–6488 (2006).
    Article CAS Google Scholar
  7. Kiga, D. et al. An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system. Proc. Natl. Acad. Sci. USA 99, 9715–9720 (2002).
    Article CAS Google Scholar
  8. Wang, L., Jackson, W.C., Steinbach, P.A. & Tsien, R.Y. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Natl. Acad. Sci. USA 101, 16745–16749 (2004).
    Article CAS Google Scholar
  9. Xie, J. & Schultz, P.G. A chemical toolkit for proteins—an expanded genetic code. Nat. Rev. Mol. Cell Biol. 7, 775–782 (2006).
    Article CAS Google Scholar
  10. Wang, L., Brock, A., Herberich, B. & Schultz, P.G. Expanding the genetic code of Escherichia coli. Science 292, 498–500 (2001).
    Article CAS Google Scholar
  11. Santoro, S.W., Anderson, J.C., Lakshman, V. & Schultz, P.G. An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli. Nucleic Acids Res. 31, 6700–6709 (2003).
    Article CAS Google Scholar
  12. Anderson, J.C. et al. An expanded genetic code with a functional quadruplet codon. Proc. Natl. Acad. Sci. USA 101, 7566–7571 (2004).
    Article CAS Google Scholar
  13. Chin, J.W. et al. An expanded eukaryotic genetic code. Science 301, 964–967 (2003).
    Article CAS Google Scholar
  14. Chin, J.W., Cropp, T.A., Chu, S., Meggers, E. & Schultz, P.G. Progress toward an expanded eukaryotic genetic code. Chem. Biol. 10, 511–519 (2003).
    Article CAS Google Scholar
  15. Wu, N., Deiters, A., Cropp, T.A., King, D. & Schultz, P.G. A genetically encoded photocaged amino acid. J. Am. Chem. Soc. 126, 14306–14307 (2004).
    Article CAS Google Scholar
  16. Summerer, D. et al. A genetically encoded fluorescent amino acid. Proc. Natl. Acad. Sci. USA 103, 9785–9789 (2006).
    Article CAS Google Scholar
  17. Wang, J., Xie, J. & Schultz, P.G. A genetically encoded fluorescent amino acid. J. Am. Chem. Soc. 128, 8738–8739 (2006).
    Article CAS Google Scholar
  18. Zhang, Z. et al. A new strategy for the synthesis of glycoproteins. Science 303, 371–373 (2004).
    Article CAS Google Scholar
  19. Liu, C. & Schultz, P.G. Recombinant expression of selectively sulfated proteins in Escherichia coli. Nat. Biotechnol. 24, 1436–1440 (2006).
    Article CAS Google Scholar
  20. Alfonta, L., Zhang, Z., Uryu, S., Loo, J.A. & Schultz, P.G. Site-specific incorporation of a redox-active amino acid into proteins. J. Am. Chem. Soc. 125, 14662–14663 (2003).
    Article CAS Google Scholar
  21. Wang, L. & Schultz, P.G. Expanding the genetic code. Angew. Chem. Int. Edn Engl. 44, 34–66 (2004).
    Article Google Scholar
  22. Bonnefond, L., Giege, R. & Rudinger-Thirion, J. Evolution of the tRNA(Tyr)/TyrRS aminoacylation systems. Biochimie 87, 873–883 (2005).
    Article CAS Google Scholar
  23. Hino, N. et al. Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid. Nat. Methods 2, 201–206 (2005).
    Article CAS Google Scholar
  24. Sprague, K.U. Transcription of eukaryotic tRNA genes (AMS Press, Washington, DC, 1994).
  25. Bedouelle, H. Recognition of tRNA(Tyr) by tyrosyl-tRNA synthetase. Biochimie 72, 589–598 (1990).
    Article CAS Google Scholar
  26. Hou, Y.M. & Schimmel, P. Modeling with in vitro kinetic parameters for the elaboration of transfer RNA identity in vivo. Biochemistry 28, 4942–4947 (1989).
    Article CAS Google Scholar
  27. Ormo, M. et al. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392–1395 (1996).
    Article CAS Google Scholar
  28. Deiters, A. et al. Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae. J. Am. Chem. Soc. 125, 11782–11783 (2003).
    Article CAS Google Scholar

Download references