Stem cell proliferative history in tissue revealed by temporal halogenated thymidine analog discrimination (original) (raw)

Nature Methods volume 2, pages 167–169 (2005)Cite this article

Abstract

Detection of proliferating cells based on bromodeoxyuridine (BrdU) incorporation and determination of phenotype by immunofluorescence labeling are standard approaches for studying stem and progenitor cell populations in developing and adult tissue as well as in histopathology studies. We describe incorporation of different halogenated thymidine analogs for temporal discrimination of cell cycle in the rat. With equimolar delivery, these analogs are suitable for quantitative histological studies including assessment of the regulation of proliferation, clonal analysis and simultaneous profiling of cell phenotype relative to proliferative history.

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Acknowledgements

This work was supported by National Institutes of Health AG20047.

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Authors and Affiliations

  1. Department of Neuroscience, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, 60064, Illinois, USA
    Christopher J Vega & Daniel A Peterson

Authors

  1. Christopher J Vega
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  2. Daniel A Peterson
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Correspondence toDaniel A Peterson.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Immunohistochemical detection can discriminate between CldU and IdU. (PDF 5294 kb)

Supplementary Fig. 2

Immunohistochemistry for CldU and IdU detects an equivalent population of proliferating cells, but only with equimolar delivery of the halogenated thymidine analogs. (PDF 4767 kb)

Supplementary Fig. 3

Temporal separation of CldU and IdU administration permits the determination of neural stem/progenitor cell proliferative history. (PDF 3317 kb)

Supplementary Methods (PDF 86 kb)

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Vega, C., Peterson, D. Stem cell proliferative history in tissue revealed by temporal halogenated thymidine analog discrimination.Nat Methods 2, 167–169 (2005). https://doi.org/10.1038/nmeth741

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