The hESC line Envy expresses high levels of GFP in all differentiated progeny (original) (raw)

Nature Methods volume 2, pages 259–260 (2005)Cite this article

Abstract

Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.

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Acknowledgements

This work was supported by Embryonic Stem Cell International (ESI) Pte. Ltd., The Australian Stem Cell Centre, The Juvenile Diabetes Research Foundation and The National Health and Medical Research Council (NHMRC) of Australia. A.G.E. is an NHMRC Senior Research Fellow. M.D. is a Howard Florey Fellow.

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Author notes

  1. Magdaline Costa and Mirella Dottori: These authors contributed equally to this work.

Authors and Affiliations

  1. Monash Immunology and Stem Cell Laboratories, STRIP 1, West Ring Road, Monash University Campus, Clayton, 3800, Victoria, Australia
    Magdaline Costa, Mirella Dottori, Elizabeth Ng, Susan M Hawes, Koula Sourris, Pegah Jamshidi, Martin F Pera, Andrew G Elefanty & Edouard G Stanley

Authors

  1. Magdaline Costa
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  2. Mirella Dottori
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  3. Elizabeth Ng
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  4. Susan M Hawes
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  5. Koula Sourris
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  6. Pegah Jamshidi
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  7. Martin F Pera
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  8. Andrew G Elefanty
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  9. Edouard G Stanley
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Corresponding author

Correspondence toEdouard G Stanley.

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Competing interests

This work was supported by Embryonic Stem Cell International (E.S.I.) Pte. Ltd. who have ownership of the Envy cell line.

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Costa, M., Dottori, M., Ng, E. et al. The hESC line Envy expresses high levels of GFP in all differentiated progeny.Nat Methods 2, 259–260 (2005). https://doi.org/10.1038/nmeth748

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