The hESC line Envy expresses high levels of GFP in all differentiated progeny (original) (raw)
- Brief Communication
- Published: 23 March 2005
- Mirella Dottori1 na1,
- Elizabeth Ng1,
- Susan M Hawes1,
- Koula Sourris1,
- Pegah Jamshidi1,
- Martin F Pera1,
- Andrew G Elefanty1 &
- …
- Edouard G Stanley1
Nature Methods volume 2, pages 259–260 (2005)Cite this article
- 1153 Accesses
- 100 Citations
- 9 Altmetric
- Metrics details
Abstract
Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Additional access options:
Similar content being viewed by others
Accession codes
Accessions
GenBank/EMBL/DDBJ
References
- Reubinoff, B.E., Pera, M.F., Fong, C.Y., Trounson, A. & Bongso, A. Nat. Biotechnol. 18, 399–404 (2000).
Article CAS Google Scholar - Stamp, L., Crosby, H.A., Hawes, S.M., Strain, A.J. & Pera, M.F. Stem Cells 23, 103–112 (2005).
Article CAS Google Scholar - Soriano, P. Nat. Genet. 21, 70–71 (1999).
Article CAS Google Scholar - Mao, X., Fujiwara, Y., Chapdelaine, A., Yang, H. & Orkin, S.H. Blood 97, 324–326 (2001).
Article CAS Google Scholar - Ma, Y., Ramezani, A., Lewis, R., Hawley, R.G. & Thomson, J.A. Stem Cells 21, 111–117 (2003).
Article CAS Google Scholar - Gropp, M. et al. Mol. Ther. 7, 281–287 (2003).
Article CAS Google Scholar - Liu, Y.P. et al. Stem Cells Dev. 13, 636–645 (2004).
Article CAS Google Scholar - Reubinoff, B.E. et al. Nat. Biotechnol. 19, 1134–1140 (2001).
Article CAS Google Scholar - Goldstein, R.S., Drukker, M., Reubinoff, B.E. & Benvenisty, N. Dev. Dyn. 225, 80–86 (2002).
Article CAS Google Scholar
Acknowledgements
This work was supported by Embryonic Stem Cell International (ESI) Pte. Ltd., The Australian Stem Cell Centre, The Juvenile Diabetes Research Foundation and The National Health and Medical Research Council (NHMRC) of Australia. A.G.E. is an NHMRC Senior Research Fellow. M.D. is a Howard Florey Fellow.
Author information
Author notes
- Magdaline Costa and Mirella Dottori: These authors contributed equally to this work.
Authors and Affiliations
- Monash Immunology and Stem Cell Laboratories, STRIP 1, West Ring Road, Monash University Campus, Clayton, 3800, Victoria, Australia
Magdaline Costa, Mirella Dottori, Elizabeth Ng, Susan M Hawes, Koula Sourris, Pegah Jamshidi, Martin F Pera, Andrew G Elefanty & Edouard G Stanley
Authors
- Magdaline Costa
You can also search for this author inPubMed Google Scholar - Mirella Dottori
You can also search for this author inPubMed Google Scholar - Elizabeth Ng
You can also search for this author inPubMed Google Scholar - Susan M Hawes
You can also search for this author inPubMed Google Scholar - Koula Sourris
You can also search for this author inPubMed Google Scholar - Pegah Jamshidi
You can also search for this author inPubMed Google Scholar - Martin F Pera
You can also search for this author inPubMed Google Scholar - Andrew G Elefanty
You can also search for this author inPubMed Google Scholar - Edouard G Stanley
You can also search for this author inPubMed Google Scholar
Corresponding author
Correspondence toEdouard G Stanley.
Ethics declarations
Competing interests
This work was supported by Embryonic Stem Cell International (E.S.I.) Pte. Ltd. who have ownership of the Envy cell line.
Supplementary information
Rights and permissions
About this article
Cite this article
Costa, M., Dottori, M., Ng, E. et al. The hESC line Envy expresses high levels of GFP in all differentiated progeny.Nat Methods 2, 259–260 (2005). https://doi.org/10.1038/nmeth748
- Received: 26 January 2005
- Accepted: 03 March 2005
- Published: 23 March 2005
- Issue Date: April 2005
- DOI: https://doi.org/10.1038/nmeth748