Fluorescent sensors for rapid monitoring of intracellular cGMP (original) (raw)

Nature Methods volume 3, pages 23–25 (2006)Cite this article

Abstract

Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.

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Acknowledgements

We thank J. Beavo, S. Cawley and W. Dostmann for plasmids, U. Walter and M. Bünemann for advice and discussion, and Bayer HealthCare AG for providing vardenafil. This work was supported by grants from the Deutsche Forschungsgemeinschaft (German Research Foundation, DFG; Leibniz award to M.J.L., Grant WA366 for S.G.) and the Fonds der Chemischen Industrie (German Chemical Industry Fund, FCI; grants to M.J.L.).

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Authors and Affiliations

  1. Institute of Pharmacology and Toxicology, Versbacher Str. 9, Würzburg, D-97078, Germany
    Viacheslav O Nikolaev & Martin J Lohse
  2. Institute of Clinical Biochemistry and Pathobiochemistry, Joseph Schneider Str. 2, Würzburg, D-97078, Germany
    Stepan Gambaryan

Authors

  1. Viacheslav O Nikolaev
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  2. Stepan Gambaryan
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  3. Martin J Lohse
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Correspondence toMartin J Lohse.

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The authors declare no competing financial interests.

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Nikolaev, V., Gambaryan, S. & Lohse, M. Fluorescent sensors for rapid monitoring of intracellular cGMP.Nat Methods 3, 23–25 (2006). https://doi.org/10.1038/nmeth816

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