Genetically encoded fluorescent indicator for intracellular hydrogen peroxide (original) (raw)

References

1. Droge, W. Free radicals in the physiological control of cell function. Physiol. Rev. 82, 47–95 (2002).
   Article CAS Google Scholar
2. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1, 145–157 (1997).
   Article CAS Google Scholar
3. Marchesi, E., Rota, C., Fann, Y.C., Chignell, C.F. & Mason, R.P. Photoreduction of the fluorescent dye 2′-7′-dichlorofluorescein: a spin trapping and direct electron spin resonance study with implications for oxidative stress measurements. Free Radic. Biol. Med. 26, 148–161 (1999).
   Article CAS Google Scholar
4. Rota, C., Fann, Y.C. & Mason, R.P. Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′,7′-dichlorofluorescein by horseradish peroxidase. Possible consequences for oxidative stress measurements. J. Biol. Chem. 274, 28161–28168 (1999).
   Article CAS Google Scholar
5. Griesbeck, O. Fluorescent proteins as sensors for cellular functions. Curr. Opin. Neurobiol. 14, 636–641 (2004).
   Article CAS Google Scholar
6. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell. Biol. 3, 906–918 (2002).
   Article CAS Google Scholar
7. Nagai, T., Sawano, A., Park, E.S. & Miyawaki, A. Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. USA 98, 3197–3202 (2001).
   Article CAS Google Scholar
8. Zheng, M., Aslund, F. & Storz, G. Activation of the OxyR transcription factor by reversible disulfide bond formation. Science 279, 1718–1721 (1998).
   Article CAS Google Scholar
9. Choi, H. et al. Structural basis of the redox switch in the OxyR transcription factor. Cell 105, 103–13 (2001).
   Article CAS Google Scholar
10. Aslund, F., Zheng, M., Beckwith, J. & Storz, G. Regulation of the OxyR transcription factor by hydrogen peroxide and the cellular thiol-disulfide status. Proc. Natl. Acad. Sci. USA 96, 6161–6165 (1999).
    Article CAS Google Scholar
11. Hausladen, A., Privalle, C.T., Keng, T., DeAngelo, J. & Stamler, J.S. Nitrosative stress: activation of the transcription factor OxyR. Cell 86, 719–729 (1996).
    Article CAS Google Scholar
12. Zorov, D.B., Filburn, C.R., Klotz, L.O., Zweier, J.L. & Sollott, S.J. Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes. J. Exp. Med. 192, 1001–1014 (2000).
    Article CAS Google Scholar
13. Thomas, W.D., Zhang, X.D., Franco, A.V., Nguyen, T. & Hersey, P. TNF-related apoptosis-inducing ligand-induced apoptosis of melanoma is associated with changes in mitochondrial membrane potential and perinuclear clustering of mitochondria. J. Immunol. 165, 5612–5620 (2000).
    Article CAS Google Scholar
14. Fulda, S., Meyer, E. & Debatin, K.M. Inhibition of TRAIL-induced apoptosis by Bcl-2 overexpression. Oncogene 21, 2283–2294 (2002).
    Article CAS Google Scholar
15. Rokhlin, O.W., Guseva, N., Tagiyev, A., Knudson, C.M. & Cohen, M.B. Bcl-2 oncoprotein protects the human prostatic carcinoma cell line PC3 from TRAIL-mediated apoptosis. Oncogene 20, 2836–2843 (2001).
    Article CAS Google Scholar
16. Walczak, H., Bouchon, A., Stahl, H. & Krammer, P.H. Tumor necrosis factor-related apoptosis-inducing ligand retains its apoptosis-inducing capacity on Bcl-2- or Bcl-xL-overexpressing chemotherapy-resistant tumor cells. Cancer Res. 60, 3051–3057 (2000).
    CAS PubMed Google Scholar
17. Keogh, S.A., Walczak, H., Bouchier-Hayes, L. & Martin, S.J. Failure of Bcl-2 to block cytochrome c redistribution during TRAIL-induced apoptosis. FEBS Lett. 471, 93–98 (2000).
    Article CAS Google Scholar
18. Suzukawa, K. et al. Nerve growth factor-induced neuronal differentiation requires generation of Rac1-regulated reactive oxygen species. J. Biol. Chem. 275, 13175–13178 (2000).
    Article CAS Google Scholar
19. Kim, S.O. et al. OxyR: a molecular code for redox-related signaling. Cell 109, 383–396 (2002).
    Article CAS Google Scholar
20. Mukhopadhyay, P., Zheng, M., Bedzyk, L.A., LaRossa, R.A. & Storz, G. Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species. Proc. Natl. Acad. Sci. USA 101, 745–750 (2004).
    Article CAS Google Scholar
21. Lee, M.W. et al. The involvement of reactive oxygen species (ROS) and p38 mitogen-activated protein (MAP) kinase in TRAIL/Apo2L-induced apoptosis. FEBS Lett. 512, 313–318 (2002).
    Article CAS Google Scholar
22. Dooley, C.T. et al. Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. J. Biol. Chem. 279, 22284–22293 (2004).
    Article CAS Google Scholar
23. Hanson, G.T. et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. J. Biol. Chem. 279, 13044–13053 (2004).
    Article CAS Google Scholar

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