A highly flexible tRNA acylation method for non-natural polypeptide synthesis (original) (raw)

Nature Methods volume 3, pages 357–359 (2006)Cite this article

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Abstract

Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.

*Note: In the version of this article initially published, the authors did not declare competing financial interests. They have filed a patent covering some of the information described in the paper and now declare competing financial interests. This error has been corrected in the PDF version of the article.

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Figure 1: Acylation of tRNA by dFx and eFx.

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Figure 2: Synthesis of a non-natural peptide by the PURE system.

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In the version of this article initially published, the authors did not declare competing financial interests. They have filed a patent covering some of the information described in the paper and now declare competing financial interests. This error has been corrected in the PDF version of the article.

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Acknowledgements

We thank M. Komiyama for the use of MALDI instrumentation. This work was supported by grants from Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (S) (16101007) and from the US National Institutes of Health (GM59159).

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Authors and Affiliations

  1. Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, 153-8904, Japan
    Hiroshi Murakami, Atsushi Ohta, Hiroshi Ashigai & Hiroaki Suga
  2. Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, 113-8656, Japan
    Atsushi Ohta, Hiroshi Ashigai & Hiroaki Suga

Authors

  1. Hiroshi Murakami
  2. Atsushi Ohta
  3. Hiroshi Ashigai
  4. Hiroaki Suga

Corresponding author

Correspondence toHiroaki Suga.

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The authors declare no competing financial interests.

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Murakami, H., Ohta, A., Ashigai, H. et al. A highly flexible tRNA acylation method for non-natural polypeptide synthesis.Nat Methods 3, 357–359 (2006). https://doi.org/10.1038/nmeth877

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