Preparation and maintenance of single-cell micro-island cultures of basal forebrain neurons (original) (raw)

References

  1. Furshpan, E.J., MacLeish, P.R., O'Lague, P.H. & Potter, D.D. Chemical transmission between rat sympathetic neurons and cardiac myocytes developing in microcultures: evidence for cholinergic, adrenergic, and dual-function neurons. Proc. Natl. Acad. Sci. USA 73, 4225–4229 (1976).
    Article CAS Google Scholar
  2. Bourque, M.J. & Trudeau, L.E. GDNF enhances the synaptic efficacy of dopaminergic neurons in culture. Eur. J. Neurosci. 12, 3172–3180 (2000).
    Article CAS Google Scholar
  3. Tarsa, L. & Goda, Y. Synaptophysin regulates activity-dependent synapse formation in cultured hippocampal neurons. Proc. Natl. Acad. Sci. USA 99, 1012–1016 (2002).
    Article CAS Google Scholar
  4. Schluter, O.M., Basu, J., Sudhof, T.C. & Rosenmund, C. Rab3 superprimes synaptic vesicles for release: implications for short-term synaptic plasticity. J. Neurosci. 26, 1239–1246 (2006).
    Article CAS Google Scholar
  5. Furshpan, E.J. Seizure-like activity in cell culture. Epilepsy Res. 10, 24–32 (1991).
    Article CAS Google Scholar
  6. Segal, M.M. Epileptiform activity in microcultures containing one excitatory hippocampal neuron. J. Neurophysiol. 65, 761–770 (1991).
    Article CAS Google Scholar
  7. Segal, M.M. Endogenous bursts underlie seizurelike activity in solitary excitatory hippocampal neurons in microcultures. J. Neurophysiol. 72, 1874–1884 (1994).
    Article CAS Google Scholar
  8. Raman, I.M., Tong, G. & Jahr, C.E. Beta-adrenergic regulation of synaptic NMDA receptors by cAMP-dependent protein kinase. Neuron 16, 415–421 (1996).
    Article CAS Google Scholar
  9. Boehm, S. & Betz, H. Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors. J. Neurosci. 17, 4066–4075 (1997).
    Article CAS Google Scholar
  10. Boehm, S. Presynaptic alpha2-adrenoceptors control excitatory, but not inhibitory, transmission at rat hippocampal synapses. J. Physiol. 519, 439–449 (1999).
    Article CAS Google Scholar
  11. Brody, D.L. & Yue, D.T. Release-independent short-term synaptic depression in cultured hippocampal neurons. J. Neurosci. 20, 2480–2494 (2000).
    Article CAS Google Scholar
  12. Tong, G., Malenka, R.C. & Nicoll, R.A. Long-term potentiation in cultures of single hippocampal granule cells: a presynaptic form of plasticity. Neuron 16, 1147–1157 (1996).
    Article CAS Google Scholar
  13. Goda, Y. & Stevens, C.F. Readily releasable pool size changes associated with long term depression. Proc. Natl. Acad. Sci. USA 95, 1283–1288 (1998).
    Article CAS Google Scholar
  14. Gomperts, S.N., Rao, A., Craig, A.M., Malenka, R.C. & Nicoll, R.A. Postsynaptically silent synapses in single neuron cultures. Neuron 21, 1443–1451 (1998).
    Article CAS Google Scholar
  15. Kimura, F., Otsu, Y. & Tsumoto, T. Presynaptically silent synapses: spontaneously active terminals without stimulus-evoked release demonstrated in cortical autapses. J. Neurophysiol. 77, 2805–2815 (1997).
    Article CAS Google Scholar
  16. Sulzer, D. et al. Dopamine neurons make glutamatergic synapses in vitro. J. Neurosci. 18, 4588–4602 (1998).
    Article CAS Google Scholar
  17. Johnson, M.D. Synaptic glutamate release by postnatal rat serotonergic neurons in microculture. Neuron 12, 433–442 (1994).
    Article CAS Google Scholar
  18. Allen, T.G., Abogadie, F.C. & Brown, D.A. Simultaneous release of glutamate and acetylcholine from single magnocellular “cholinergic” basal forebrain neurons. J. Neurosci. 26, 1588–1595 (2006).
    Article CAS Google Scholar
  19. Joyce, M.P. & Rayport, S. Mesoaccumbens dopamine neuron synapses reconstructed in vitro are glutamatergic. Neuroscience 99, 445–456 (2000).
    Article CAS Google Scholar
  20. Kimura, F. & Baughman, R.W. Distinct muscarinic receptor subtypes suppress excitatory and inhibitory synaptic responses in cortical neurons. J. Neurophysiol. 77, 709–716 (1997).
    Article CAS Google Scholar
  21. Bekkers, J.M. & Stevens, C.F. Excitatory and inhibitory autaptic currents in isolated hippocampal neurons maintained in cell culture. Proc. Natl. Acad. Sci. USA 88, 7834–7838 (1991).
    Article CAS Google Scholar
  22. Mennerick, S., Que, J., Benz, A. & Zorumski, C.F. Passive and synaptic properties of hippocampal neurons grown in microcultures and in mass cultures. J. Neurophysiol. 73, 320–332 (1995).
    Article CAS Google Scholar
  23. Ullian, E.M., Christopherson, K.S. & Barres, B.A. Role for glia in synaptogenesis. Glia 47, 209–216 (2004).
    Article Google Scholar
  24. Brewer, G.J., Torricelli, J.R., Evege, E.K. & Price, P.J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J. Neurosci. Res. 35, 567–576 (1993).
    Article CAS Google Scholar
  25. Brewer, G.J. Serum-free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus. J. Neurosci. Res. 42, 674–683 (1995).
    Article CAS Google Scholar
  26. Segal, M.M., Baughman, R.W., Jones, K.A. & Huettner, J.E. Mass cultures and microislands of neurons from postnatal rat brain. in Culturing Nerve Cells 2nd edn. (eds. Banker, G. & Goslin, K.) 309–338 (MIT, Cambridge, Massachusetts).
  27. Wainer, B.H. & Mesulam, M.-M. Ascending cholinergic pathways in the rat brain. in Brain Cholinergic Systems (eds. Steriade, M. & Biesold, D.) 65–119 (Oxford University Press, New York, USA).
  28. Segal, M.M. & Furshpan, E.J. Epileptiform activity in microcultures containing small numbers of hippocampal neurons. J. Neurophysiol. 64, 1390–1399 (1990).
    Article CAS Google Scholar
  29. Huettner, J.E. & Baughman, R.W. The pharmacology of synapses formed by identified corticocollicular neurons in primary cultures of rat visual cortex. J. Neurosci. 8, 160–175 (1998).
    Article Google Scholar
  30. Chen, G. & van den Pol, A.N. Multiple NPY receptors coexist in pre- and postsynaptic sites: inhibition of GABA release in isolated self-inervating SCN neurons. J. Neurosci. 16, 7711–7724 (1996).
    Article CAS Google Scholar
  31. Radcliffe, k.A. & Dani, J.A. Nicotinic stimulation produces multiple forms of increased glutamatergic synaptic transmission. J. Neurosci. 18, 7075–7083 (1998).
    Article CAS Google Scholar
  32. Pyott, S.J. & Rosenmund, C. The effects of temperature on vesicular supply and release in autaptic cultures of rat and mouse hippocampal neurons. J. Physiol. 539, 523–535 (2002).
    Article CAS Google Scholar
  33. Nagler, K., Mauch, D.H. & Pfrieger, F.W. Glia-derived signals induce synapse formation in neurones of the rat central nervous system. J. Physiol. 533, 665–679 (2001).
    Article CAS Google Scholar

Download references