Site-specific protein labeling by Sfp phosphopantetheinyl transferase (original) (raw)

References

1. Lambalot, R.H. et al. A new enzyme superfamily—the phosphopantetheinyl transferases. Chem. Biol. 3, 923–936 (1996).
   CAS PubMed Google Scholar
2. Walsh, C.T., Gehring, A.M., Weinreb, P.H., Quadri, L.E. & Flugel, R.S. Post-translational modification of polyketide and nonribosomal peptide synthases. Curr. Opin. Chem. Biol. 1, 309–315 (1997).
   CAS PubMed Google Scholar
3. Belshaw, P.J., Walsh, C.T. & Stachelhaus, T. Aminoacyl-CoAs as probes of condensation domain selectivity in nonribosomal peptide synthesis. Science 284, 486–489 (1999).
   CAS PubMed Google Scholar
4. La Clair, J.J., Foley, T.L., Schegg, T.R., Regan, C.M. & Burkart, M.D. Manipulation of carrier proteins in antibiotic biosynthesis. Chem. Biol. 11, 195–201 (2004).
   CAS PubMed Google Scholar
5. Sieber, S.A., Walsh, C.T. & Marahiel, M.A. Loading peptidyl-coenzyme A onto peptidyl carrier proteins: a novel approach in characterizing macrocyclization by thioesterase domains. J. Am. Chem. Soc. 125, 10862–10866 (2003).
   CAS PubMed Google Scholar
6. Vitali, F., Zerbe, K. & Robinson, J.A. Production of vancomycin aglycone conjugated to a peptide carrier domain derived from a biosynthetic non-ribosomal peptide synthetase. Chem. Commun. (Camb) 21, 2718–2719 (2003).
   Google Scholar
7. Flugel, R.S., Hwangbo, Y., Lambalot, R.H., Cronan, J.E., Jr. & Walsh, C.T. Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli. J. Biol. Chem. 275, 959–968 (2000).
   CAS PubMed Google Scholar
8. Gehring, A.M., Lambalot, R.H., Vogel, K.W., Drueckhammer, D.G. & Walsh, C.T. Ability of Streptomyces spp. acyl carrier proteins and coenzyme A analogs to serve as substrates in vitro for E. coli holo-ACP synthase. Chem. Biol. 4, 17–24 (1997).
   CAS PubMed Google Scholar
9. Mofid, M.R., Finking, R. & Marahiel, M.A. Recognition of hybrid peptidyl carrier proteins/acyl carrier proteins in nonribosomal peptide synthetase modules by the 4′-phosphopantetheinyl transferases AcpS and Sfp. J. Biol. Chem. 277, 17023–17031 (2002).
   CAS PubMed Google Scholar
10. George, N., Pick, H., Vogel, H., Johnsson, N. & Johnsson, K. Specific labeling of cell surface proteins with chemically diverse compounds. J. Am. Chem. Soc. 126, 8896–8897 (2004).
    CAS PubMed Google Scholar
11. Yin, J., Liu, F., Li, X. & Walsh, C.T. Labeling proteins with small molecules by site-specific posttranslational modification. J. Am. Chem. Soc. 126, 7754–7755 (2004).
    CAS PubMed Google Scholar
12. Yin, J., Liu, F., Schinke, M., Daly, C. & Walsh, C.T. Phagemid encoded small molecules for high throughput screening of chemical libraries. J. Am. Chem. Soc. 126, 13570–13571 (2004).
    CAS PubMed Google Scholar
13. Vivero-Pol, L., George, N., Krumm, H., Johnsson, K. & Johnsson, N. Multicolor imaging of cell surface proteins. J. Am. Chem. Soc. 127, 12770–12771 (2005).
    CAS PubMed Google Scholar
14. Yin, J. et al. Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp catalyzed site specific protein labeling. Chem. Biol. 12, 999–1006 (2005).
    CAS PubMed PubMed Central Google Scholar
15. Yin, J. et al. Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proc. Natl. Acad. Sci. USA 102, 15815–15820 (2005).
    CAS PubMed Google Scholar
16. Hermanson, G.T. Bioconjugate Techniques (Academic Press, San Diego, 1995), p. 148.
    Google Scholar
17. Stachelhaus, T. & Walsh, C.T. Mutational analysis of the epimerization domain in the initiation module PheATE of gramicidin S synthetase. Biochemistry 39, 5775–5787 (2000).
    CAS PubMed Google Scholar
18. Horton, R.M. et al. Gene splicing by overlap extension. Methods Enzymol. 217, 270–279 (1993).
    CAS PubMed Google Scholar
19. McGraw, T.E., Greenfield, L. & Maxfield, F.R. Functional expression of the human transferrin receptor cDNA in Chinese hamster ovary cells deficient in endogenous transferrin receptor. J. Cell. Biol. 105, 207–214 (1987).
    CAS PubMed Google Scholar

Download references