The comet assay: a method to measure DNA damage in individual cells (original) (raw)
Ostling, O. & Johanson, K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun.123, 291–298 (1984). ArticleCASPubMed Google Scholar
Singh, N.P., McCoy, M.T., Tice, R.R. & Schneider, E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res.175, 184–191 (1988). ArticleCAS Google Scholar
Rydberg, B. The rate of strand separation in alkali of DNA of irradiated mammalian cells. Radiat. Res.61, 274–287 (1975). ArticleCASPubMed Google Scholar
Kohn, K.W. & Grimek-Ewig, R.A. Alkaline elution analysis, a new approach to the study of DNA single-strand interruptions in cells. Cancer Res.33, 1849–1853 (1973). CASPubMed Google Scholar
Olive, P.L. Cell proliferation as a requirement for development of the contact effect in Chinese hamster V79 spheroids. Radiat. Res.117, 79–92 (1989). ArticleCASPubMed Google Scholar
Olive, P.L., Banáth, J.P. & Durand, R.E. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the “comet” assay. Radiat. Res.122, 86–94 (1990).
Fairbairn, D.W., Olive, P.L. & O'Neill, K.L. The comet assay: a comprehensive review. Mutat. Res.339, 37–59 (1995). ArticleCASPubMed Google Scholar
Salagovic, J., Gilles, J., Verschaeve, L. & Kalina, I. The comet assay for the detection of genotoxic damage in the earthworms: a promising tool for assessing the biological hazards of polluted sites. Folia Biol. (Praha.)42, 17–21 (1996). CAS Google Scholar
Moller, P. Genotoxicity of environmental agents assessed by the alkaline comet assay. Basic Clin. Pharmacol. Toxicol.96 (Suppl. 1), 1–42 (2005). CASPubMed Google Scholar
Kiskinis, E., Suter, W. & Hartmann, A. High-throughput Comet assay using 96-well plates. Mutagenesis17, 37–43 (2002). ArticleCASPubMed Google Scholar
Frieauff, W., Hartmann, A. & Suter, W. Automatic analysis of slides processed in the Comet assay. Mutagenesis16, 133–137 (2001). ArticleCASPubMed Google Scholar
Tice, R.R. et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen.35, 206–221 (2000). ArticleCASPubMed Google Scholar
Bauch, T., Bocker, W., Mallek, U., Muller, W.U. & Streffer, C. Optimization and standardization of the “comet assay” for analyzing the repair of DNA damage in cells. Strahlenther. Onkol.175, 333–340 (1999). ArticleCASPubMed Google Scholar
Banath, J.P., Kim, A. & Olive, P.L. Overnight lysis improves the efficiency of detection of DNA damage in the alkaline comet assay. Radiat. Res.155, 564–571 (2001). ArticleCASPubMed Google Scholar
Olive, P.L., Wlodek, D. & Banáth, J.P. DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Res.51, 4671–4676 (1991). CASPubMed Google Scholar
Collins, A.R., Duthie, S.J. & Dobson, V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogen.14, 1733–1735 (1993). ArticleCAS Google Scholar
Merk, O. & Speit, G. Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity. Environ. Mol. Mutagen.33, 167–172 (1999). ArticleCASPubMed Google Scholar
Olive, P.L., Frazer, G. &p Banáth, J.P. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res.136, 130–136 (1993). ArticleCASPubMed Google Scholar
Ostling, O. & Johanson, K.J. Bleomycin, in contrast to gamma irradiation, induces extreme variation of DNA strand breakage from cell to cell. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med.52, 683–691 (1987). ArticleCASPubMed Google Scholar
Olive, P.L., Johnston, P.J., Banáth, J.P. & Durand, R.E. The comet assay: A new method to examine heterogeneity associated with solid tumors. Nat. Med.4, 103–105 (1998). ArticleCASPubMed Google Scholar
Olive, P.L., Durand, R.E., LeRiche, J.C., Olivotto, I.A. & Jackson, S.M. Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers. Cancer Res.53, 733–736 (1993). CASPubMed Google Scholar
Olive, P.L. & Banáth, J.P. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res.57, 5528–5533 (1997). CASPubMed Google Scholar
Almeida, G.M., Duarte, T.L., Steward, W.P. & Jones, G.D. Detection of oxaliplatin-induced DNA crosslinks in vitro and in cancer patients using the alkaline comet assay. DNA Repair (Amst.)5, 219–225 (2006). ArticleCAS Google Scholar
Dorie, M.J. et al. DNA damage measured by the comet assay in head and neck cancer patients treated with tirapazamine. Neoplasia.1, 461–467 (1999). ArticleCASPubMedPubMed Central Google Scholar
Speit, G. & Hartmann, A. The comet assay: a sensitive genotoxicity test for the detection of DNA damage. Methods Mol. Biol.291, 85–95 (2005). CASPubMed Google Scholar
Strauss, G.H., Peters, W.P. & Everson, R.B. Measuring DNA damage in individual cells of heterogeneous mixtures: a novel application of an immunological typing technique. Mutat. Res.304, 211–216 (1994). ArticleCASPubMed Google Scholar
Santos, S.J., Singh, N.P. & Natarajan, A.T. Fluorescence in situ hybridization with comets. Exp. Cell Res.232, 407–411 (1997). ArticleCASPubMed Google Scholar
Rapp, A., Hausmann, M. & Greulich, K.O. The comet-FISH technique: a tool for detection of specific DNA damage and repair. Methods Mol. Biol.291, 107–119 (2005). CASPubMed Google Scholar
Speit, G. & Hartmann, A. The comet assay: a sensitive genotoxicity test for the detection of DNA damage. Methods Mol. Biol.291, 85–95 (2005). CASPubMed Google Scholar
Hartmann, A. et al. Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. Mutagenesis18, 45–51 (2003). ArticleCASPubMed Google Scholar
Albertini, R.J. et al. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. Mutat. Res.463, 111–172 (2000). ArticleCASPubMed Google Scholar
Duez, P., Dehon, G., Kumps, A. & Dubois, J. Statistics of the Comet assay: a key to discriminate between genotoxic effects. Mutagenesis18, 159–166 (2003). ArticleCASPubMed Google Scholar
Verde, P.E. et al. Application of public-domain statistical analysis software for evaluation and comparison of comet assay data. Mutat. Res.604, 71–82 (2006). ArticleCASPubMed Google Scholar
Olive, P.L. & Durand, R.E. Heterogeneity in DNA damage using the comet assay. Cytometry A66, 1–8 (2005). ArticlePubMed Google Scholar
Olive, P.L. & Johnston, P.J. DNA damage from oxidants: influence of lesion complexity and chromatin organization. Oncol. Res.9, 287–294 (1997). CASPubMed Google Scholar
Sasaki, Y.F. et al. The comet assay with multiple mouse organs: comparison of comet assay results and carcinogenicity with 208 chemicals selected from the IARC monographs and U.S. NTP Carcinogenicity Database. Crit. Rev. Toxicol.30, 629–799 (2000). ArticleCASPubMed Google Scholar
Cedervall, B. et al. Methods for the quantification of DNA double-strand breaks determined from the distribution of DNA fragment sizes measured by pulsed-field gel electrophoresis. Radiat. Res.143, 8–16 (1995). ArticleCASPubMed Google Scholar
Olive, P.L. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol.75, 395–405 (1999). ArticleCASPubMed Google Scholar
Speit, G. & Hartmann, A. The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay). Mutagenesis10, 555–559 (1995). ArticleCASPubMed Google Scholar
Alapetite, C., Wachter, T., Sage, E. & Moustacchi, E. Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and γ rays. Int. J. Radiat. Biol.69, 359–369 (1996). ArticleCASPubMed Google Scholar
Rydberg, B. Radiation-induced heat-labile sites that convert into DNA double-strand breaks. Radiat. Res.153, 805–812 (2000). ArticleCASPubMed Google Scholar
Collins, A.R. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol. Biotechnol.26, 249–261 (2004). ArticleCASPubMed Google Scholar
Olive, P.L., Banáth, J.P. & Fjell, C.D. DNA strand breakage and DNA structure influence staining with propidium iodide using the alkaline comet assay. Cytometry16, 305–312 (1994). ArticleCASPubMed Google Scholar