In vitro screens for quorum sensing inhibitors and in vivo confirmation of their effect (original) (raw)

References

  1. Fuqua, W.C., Winans, S.C. & Greenberg, E.P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J. Bacteriol. 176, 269–275 (1994).
    Article CAS PubMed PubMed Central Google Scholar
  2. Hentzer, M. et al. Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. EMBO J. 22, 3803–3815 (2003).
    Article CAS PubMed PubMed Central Google Scholar
  3. Bjarnsholt, T. et al. Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology 151, 3873–3880 (2005).
    Article CAS PubMed Google Scholar
  4. Bjarnsholt, T. et al. Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent. Microbiology 151, 373–383 (2005).
    Article CAS PubMed Google Scholar
  5. Christensen, L.D. et al. Impact of Pseudomonas aeruginosa quorum sensing on biofilm persistence in an in vivo intraperitoneal foreign-body infection model. Microbiology 153, 2312–2320 (2007).
    Article CAS PubMed Google Scholar
  6. Jensen, P.O. et al. Rapid necrotic killing of polymorphonuclear leukocytes is caused by quorum-sensing-controlled production of rhamnolipid by Pseudomonas aeruginosa . Microbiology 153, 1329–1338 (2007).
    Article CAS PubMed Google Scholar
  7. Costerton, J.W., Stewart, P.S. & Greenberg, E.P. Bacterial biofilms: a common cause of persistent infections. Science 284, 1318–1322 (1999).
    Article CAS PubMed Google Scholar
  8. Kirketerp-Moller, K. et al. Distribution, organization, and ecology of bacteria in chronic wounds. J Clin. Microbiol. 46, 2717–2722 (2008).
    Article PubMed PubMed Central Google Scholar
  9. Homoe, P., Bjarnsholt, T., Wessman, M., Sorensen, H.C. & Johansen, H.K. Morphological evidence of biofilm formation in Greenlanders with chronic suppurative otitis media. Eur. Arch. Otorhinolaryngol. (2009).
  10. Bjarnsholt, T., Tolker-Nielsen, T., Givskov, M., Janssen, M. & Chrsitensen, L. Detection of bacteria by FISH in culture-negative soft tissue filler lesions. Dermatol. Surg. in press. (2009).
  11. Bjarnsholt, T. et al. Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. Pediatr. Pulmonol. 44, 547–558 (2009).
    Article PubMed Google Scholar
  12. Neut, D., van, H., Jr., van Kooten, T.G., van der Mei, H.C. & Busscher, H.J. Detection of biomaterial-associated infections in orthopaedic joint implants. Clin. Orthop. Relat. Res. 261–268 (2003).
  13. Givskov, M. et al. Eukaryotic interference with homoserine lactone-mediated prokaryotic signalling. J. Bacteriol. 178, 6618–6622 (1996).
    Article CAS PubMed PubMed Central Google Scholar
  14. Wu, H. et al. Synthetic furanones inhibit quorum-sensing and enhance bacterial clearance in Pseudomonas aeruginosa lung infection in mice. J. Antimicrob. Chemother. 53, 1054–1061 (2004).
    Article CAS PubMed Google Scholar
  15. Rasmussen, T.B. et al. Identity and effects of quorum sensing inhibitors produced by Penicillum species. Microbiology 151, 1325–1340 (2005).
    Article CAS PubMed Google Scholar
  16. Skindersoe, M.E. et al. Quorum sensing antagonism from marine organisms. Mar. Biotechnol. (NY) 10, 56–63 (2008).
    Article CAS Google Scholar
  17. Rasmussen, T.B. et al. Screening for quorum sensing inhibitors using a novel genetic system—the QSI selector. J. Bacteriol. 187, 1799–1814 (2005).
    Article CAS PubMed PubMed Central Google Scholar
  18. Skindersoe, M.E. et al. Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa . Antimicrob. Agents Chemother. 52, 3648–3663 (2008).
    Article CAS PubMed PubMed Central Google Scholar
  19. Yang, L. et al. Computer-aided identification of recognized drugs as Pseudomonas aeruginosa quorum-sensing inhibitors. Antimicrob. Agents Chemother. 53, 2432–2443 (2009).
    Article CAS PubMed PubMed Central Google Scholar
  20. Ravn, L., Christensen, A.B., Molin, S., Givskov, M. & Gram, L. Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics. J Microbiol. Methods 44, 239–251 (2001).
    Article CAS PubMed Google Scholar
  21. Hentzer, M. et al. Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology 148, 87–102 (2002).
    Article CAS PubMed Google Scholar
  22. Pedersen, S.S., Shand, G.H., Hansen, B.L. & Hansen, G.N. Induction of experimental chronic Pseudomonas aeruginosa lung infection with P. aeruginosa entrapped in alginate microspheres. APMIS 98, 203–211 (1990).
    Article CAS PubMed Google Scholar
  23. Wu, H. et al. Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa . Microbiology 146 (Pt 10): 2481–2493 (2000).
    Article CAS PubMed Google Scholar
  24. Buret, A., Ward, K.H., Olson, M.E. & Costerton, J.W. An in vivo model to study the pathobiology of infectious biofilms on biomaterial surfaces. J. Biomed. Mater. Res. 25, 865–874 (1991).
    Article CAS PubMed Google Scholar
  25. van, G.M. et al. Inactivation of the rhlA gene in Pseudomonas aeruginosa prevents rhamnolipid production, disabling the protection against polymorphonuclear leukocytes. APMIS 117, 537–546 (2009).
    Article Google Scholar
  26. Kohler, T., van Delden, C., Curty, L.K., Hamzehpour, M.M. & Pechere, J.C. Overexpression of the MexEF-OprN multidrug efflux system affects cell-to-cell signaling in Pseudomonas aeruginosa . J. Bacteriol. 183, 5213–5222 (2001).
    Article CAS PubMed PubMed Central Google Scholar
  27. Høiby, N. Epidemiological investigations of the respiratory tract bacteriology in patients with cystic fibrosis. Acta. Pathol. Microbiol. Scand. B Microbiol. Immunol. 82, 541–550 (1974).
    PubMed Google Scholar

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