In vivo two-photon imaging of sensory-evoked dendritic calcium signals in cortical neurons (original) (raw)

References

  1. London, M. & Häusser, M. Dendritic computation. Annu. Rev. Neurosci. 28, 503–532 (2005).
    Article CAS Google Scholar
  2. Johnston, D. & Narayanan, R. Active dendrites: colorful wings of the mysterious butterflies. Trends. Neurosci. 31, 309–316 (2008).
    Article CAS Google Scholar
  3. Larkum, M.E. & Nevian, T. Synaptic clustering by dendritic signalling mechanisms. Curr. Opin. Neurobiol. 18, 321–331 (2008).
    Article CAS Google Scholar
  4. Ohki, K. & Reid, R.C. Specificity and randomness in the visual cortex. Curr. Opin. Neurobiol. 17, 401–407 (2007).
    Article CAS Google Scholar
  5. Bloodgood, B.L. & Sabatini, B.L. Ca2+ signaling in dendritic spines. Curr. Opin. Neurobiol. 17, 345–351 (2007).
    Article CAS Google Scholar
  6. Yuste, R. & Denk, W. Dendritic spines as basic functional units of neuronal integration. Nature 375, 682–684 (1995).
    Article CAS Google Scholar
  7. Müller, W. & Connor, J.A. Dendritic spines as individual neuronal compartments for synaptic Ca2+ responses. Nature 354, 73–76 (1991).
    Article Google Scholar
  8. Eilers, J., Augustine, G.J. & Konnerth, A. Subthreshold synaptic Ca2+ signalling in fine dendrites and spines of cerebellar Purkinje neurons. Nature 373, 155–158 (1995).
    Article CAS Google Scholar
  9. Häusser, M. & Mel, B. Dendrites: bug or feature? Curr. Opin. Neurobiol. 13, 372–383 (2003).
    Article Google Scholar
  10. Svoboda, K., Denk, W., Kleinfeld, D. & Tank, D.W. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385, 161–165 (1997).
    Article CAS Google Scholar
  11. Helmchen, F., Svoboda, K., Denk, W. & Tank, D.W. In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons. Nat. Neurosci. 2, 989–996 (1999).
    Article CAS Google Scholar
  12. Murayama, M. et al. Dendritic encoding of sensory stimuli controlled by deep cortical interneurons. Nature 457, 1137–1141 (2009).
    Article CAS Google Scholar
  13. Waters, J., Larkum, M., Sakmann, B. & Helmchen, F. Supralinear Ca2+ influx into dendritic tufts of layer 2/3 neocortical pyramidal neurons in vitro and in vivo. J. Neurosci. 23, 8558–8567 (2003).
    Article CAS Google Scholar
  14. Waters, J. & Helmchen, F. Boosting of action potential backpropagation by neocortical network activity in vivo. J. Neurosci. 24, 11127–11136 (2004).
    Article CAS Google Scholar
  15. Svoboda, K., Helmchen, F., Denk, W. & Tank, D.W. Spread of dendritic excitation in layer 2/3 pyramidal neurons in rat barrel cortex in vivo. Nat. Neurosci. 2, 65–73 (1999).
    Article CAS Google Scholar
  16. Helmchen, F. & Waters, J. Ca2+ imaging in the mammalian brain in vivo. Eur. J. Pharmacol. 447, 119–129 (2002).
    Article CAS Google Scholar
  17. Jia, H., Rochefort, N.L., Chen, X. & Konnerth, A. Dendritic organization of sensory input to cortical neurons in vivo. Nature 464, 1307–1312 (2010).
    Article CAS Google Scholar
  18. Kitamura, K., Judkewitz, B., Kano, M., Denk, W. & Häusser, M. Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo. Nat. Methods 5, 61–67 (2008).
    Article CAS Google Scholar
  19. Bollmann, J.H. & Engert, F. Subcellular topography of visually driven dendritic activity in the vertebrate visual system. Neuron 61, 895–905 (2009).
    Article CAS Google Scholar
  20. Margrie, T.W., Brecht, M. & Sakmann, B. In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain. Pflugers Arch. 444, 491–498 (2002).
    Article CAS Google Scholar
  21. Nevian, T. & Helmchen, F. Calcium indicator loading of neurons using single-cell electroporation. Pflugers Arch. 454, 675–688 (2007).
    Article CAS Google Scholar
  22. Nagayama, S. et al. In vivo simultaneous tracing and Ca2+ imaging of local neuronal circuits. Neuron 53, 789–803 (2007).
    Article CAS Google Scholar
  23. Theer, P., Hasan, M.T. & Denk, W. Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier. Opt. Lett. 28, 1022–1024 (2003).
    Article CAS Google Scholar
  24. Jung, J.C. & Schnitzer, M.J. Multiphoton endoscopy. Opt. Lett. 28, 902–904 (2003).
    Article Google Scholar
  25. Mank, M. & Griesbeck, O. Genetically encoded calcium indicators. Chem. Rev. 108, 1550–1564 (2008).
    Article CAS Google Scholar
  26. Hires, S.A., Tian, L. & Looger, L.L. Reporting neural activity with genetically encoded calcium indicators. Brain Cell. Biol. 36, 69–86 (2008).
    Article CAS Google Scholar
  27. Miyawaki, A. Fluorescence imaging of physiological activity in complex systems using GFP-based probes. Curr. Opin. Neurobiol. 13, 591–596 (2003).
    Article CAS Google Scholar
  28. Lutcke, H. et al. Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice. Front. Neural. Circuits 4, 9 (2010).
    PubMed PubMed Central Google Scholar
  29. Tian, L. et al. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat. Methods 6, 875–881 (2009).
    Article CAS Google Scholar
  30. Rochefort, N.L. & Konnerth, A. Genetically encoded Ca2+ sensors come of age. Nat. Methods 5, 761–762 (2008).
    Article CAS Google Scholar
  31. Niell, C.M. & Stryker, M.P. Highly selective receptive fields in mouse visual cortex. J. Neurosci. 28, 7520–7536 (2008).
    Article CAS Google Scholar

Download references