The structural basis of transferrin sequestration by transferrin-binding protein B (original) (raw)

Nature Structural & Molecular Biology volume 19, pages 358–360 (2012)Cite this article

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Abstract

Neisseria meningitidis, the causative agent of bacterial meningitis, acquires the essential element iron from the host glycoprotein transferrin during infection through a surface transferrin receptor system composed of proteins TbpA and TbpB. Here we present the crystal structures of TbpB from N. meningitidis in its apo form and in complex with human transferrin. The structure reveals how TbpB sequesters and initiates iron release from human transferrin.

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Acknowledgements

We thank members of the Advanced Photon Source at both of the Northeastern Collaborative Access Team's beamlines 24-ID-E and 24-ID-C, the Canadian Light Source beamline staff at the Canadian Macromolecular Crystallography Facility CMCF-08ID-1 for assistance with data collection, and members of the Moraes and Schryvers laboratories for valuable discussions. This research was funded with operating and infrastructure support provided by the Canadian Foundation for Innovation, the Alberta Innovates Health Solutions and the Canadian Institutes of Health Research.

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Authors and Affiliations

  1. Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    Charles Calmettes & Trevor F Moraes
  2. Department of Microbiology & Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    Joenel Alcantara, Rong-Hua Yu & Anthony B Schryvers
  3. Department of Biochemistry & Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    Joenel Alcantara, Rong-Hua Yu & Anthony B Schryvers

Authors

  1. Charles Calmettes
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  2. Joenel Alcantara
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  3. Rong-Hua Yu
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  4. Anthony B Schryvers
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  5. Trevor F Moraes
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Contributions

C.C., A.B.S. and T.F.M. conceived and designed the experiments, C.C. conducted experiments and data analysis, J.A. provided the wild-type _Nm_M982-TbpB–containing plasmid, R.-H.Y. provided deglycosylated human transferrin and C.C. and T.F.M. wrote the manuscript. All authors read and commented on the draft versions of the manuscript and approved the final version.

Corresponding author

Correspondence toTrevor F Moraes.

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The authors declare no competing financial interests.

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Calmettes, C., Alcantara, J., Yu, RH. et al. The structural basis of transferrin sequestration by transferrin-binding protein B.Nat Struct Mol Biol 19, 358–360 (2012). https://doi.org/10.1038/nsmb.2251

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