The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase (original) (raw)

Nature Structural & Molecular Biology volume 14, pages 344–346 (2007)Cite this article

Abstract

Histone H3 Lys4 (H3K4) is methylated by yeast Set1–COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.

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Acknowledgements

We thank R. Perry (Fox Chase Cancer Center) for the Chd1 antiserum. J.C.E. was supported by grants from the US National Science Foundation (MCB 0131414 and MCB 0615831). R.S. was supported by a grant from the US National Institutes of Health (GM61204). A.S. was supported by grants from the US National Institutes of Health (CA089455 and GM069905).

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Author notes

  1. Joel C Eissenberg and Min Gyu Lee: These authors contributed equally to this manuscript.

Authors and Affiliations

  1. Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, 63104, Missouri, USA
    Joel C Eissenberg, Jessica Schneider, Anne Ilvarsonn & Ali Shilatifard
  2. Saint Louis University Cancer Center, Saint Louis University School of Medicine, St. Louis, 63104, Missouri, USA
    Joel C Eissenberg & Ali Shilatifard
  3. The Wistar Institute, 3601 Spruce Street, Philadelphia, 19104, Pennsylvania, USA
    Min Gyu Lee & Ramin Shiekhattar
  4. Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, 64110, Missouri, USA
    Ali Shilatifard

Authors

  1. Joel C Eissenberg
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  2. Min Gyu Lee
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  3. Jessica Schneider
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  4. Anne Ilvarsonn
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  5. Ramin Shiekhattar
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  6. Ali Shilatifard
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Correspondence toAli Shilatifard.

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Eissenberg, J., Lee, M., Schneider, J. et al. The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase.Nat Struct Mol Biol 14, 344–346 (2007). https://doi.org/10.1038/nsmb1217

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