The stalk region of dynamin drives the constriction of dynamin tubes (original) (raw)

Nature Structural & Molecular Biology volume 11, pages 574–575 (2004)Cite this article

Abstract

The GTPase dynamin is essential for numerous vesiculation events including clathrin-mediated endocytosis. Upon GTP hydrolysis, dynamin constricts a lipid bilayer. Previously, a three-dimensional structure of mutant dynamin in the constricted state was determined by helical reconstruction methods. We solved the nonconstricted state by a single-particle approach and show that the stalk region of dynamin undergoes a large conformational change that drives tube constriction.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 12 print issues and online access

$209.00 per year

only $17.42 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Hinshaw, J.E. Annu. Rev. Cell Dev. Biol. 16, 483–519 (2000).
    Article CAS Google Scholar
  2. Danino, D. & Hinshaw, J.E. Curr. Opin. Cell Biol. 13, 454–460 (2001).
    Article CAS Google Scholar
  3. Song, B.D. & Schmid, S.L. Biochemistry 42, 1369–1376 (2003).
    Article CAS Google Scholar
  4. Smirnova, E., Shurland, D.L., Newman-Smith, E.D., Pishvaee, B. & van der Bliek, A.M. J. Biol. Chem. 274, 14942–14947 (1999).
    Article CAS Google Scholar
  5. Sweitzer, S.M. & Hinshaw, J.E. Cell 93, 1021–1029 (1998).
    Article CAS Google Scholar
  6. Zhang, P. & Hinshaw, J.E. Nat. Cell Biol. 3, 922–926 (2001).
    Article CAS Google Scholar
  7. Danino, D., Moon, K. & Hinshaw, J.E. J. Struct. Biol. in the press.
  8. Egelman, E.H. Ultramicroscopy 85, 225–234 (2000).
    Article CAS Google Scholar
  9. Okamoto, P.M., Tripet, B., Litowski, J., Hodges, R.S. & Vallee, R.B. J. Biol. Chem. 274, 10277–10286 (1999).
    Article CAS Google Scholar
  10. Eccleston, J.F., Binns, D.D., Davis, C.T., Albanesi, J.P. & Jameson, D.M. Eur. Biophys. J. 31, 275–282 (2002).
    Article CAS Google Scholar
  11. Warnock, D.E., Hinshaw, J.E. & Schmid, S.L. J. Biol. Chem. 271, 22310–22314 (1996).
    Article CAS Google Scholar
  12. Stowell, M.H., Marks, B., Wigge, P. & McMahon, H.T. Nat. Cell Biol. 1, 27–32 (1999).
    Article CAS Google Scholar

Download references

Acknowledgements

We thank K.-H. Moon for his help with the helical hand determination. This work was supported by a grant from the US National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health, to E.H.E.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA
    Yen-Ju Chen & Edward H Egelman
  2. Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Peijun Zhang & Jenny E Hinshaw

Authors

  1. Yen-Ju Chen
    You can also search for this author inPubMed Google Scholar
  2. Peijun Zhang
    You can also search for this author inPubMed Google Scholar
  3. Edward H Egelman
    You can also search for this author inPubMed Google Scholar
  4. Jenny E Hinshaw
    You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toEdward H Egelman.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

About this article

Cite this article

Chen, YJ., Zhang, P., Egelman, E. et al. The stalk region of dynamin drives the constriction of dynamin tubes.Nat Struct Mol Biol 11, 574–575 (2004). https://doi.org/10.1038/nsmb762

Download citation