Anti-K-ras ribozyme induces growth inhibition and increased chemosensitivity in human colon cancer cells (original) (raw)

Cancer Gene Therapy volume 7, pages 495–500 (2000)Cite this article

Abstract

Colon cancer is one of the carcinomas that is resistant to a variety of therapies. To develop a new therapy by regulating the activated K-ras gene in colon cancers, we prepared synthetic DNA encoding the ribozyme (catalytic RNA), and inserted it into an expression vector (LNCX) originated from a retrovirus. Transfection of the vector into SW620 human colon cancer cells brought about significant suppression of K-ras mRNA synthesis and inhibition of the cell growth. Studies in athymic mice, in which K-ras ribozyme-introduced SW620 cells were transplanted, also revealed a marked reduction of tumor growth in vivo. Furthermore, the ribozyme-introduced tumors became more sensitive to treatment with anti-cancer drugs such as cisplatin, adriamycin, 5-fluorouracil, vincristine, and etoposide. These data suggest that the possible efficacy of anti-K-ras ribozyme increases the chemosensitivity of human colon cancers as well as the inhibitory effect on the growth of human colon cancers.

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Authors and Affiliations

  1. Department of Molecular Diagnostics, Tohoku University School of Medicine, Sendai, Japan
    Tadao Funato, Tomonori Ishii & Takeshi Sasaki
  2. Department of Clinical Oncology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan
    Mariko Kambe
  3. Berlex Biosciences, Richmond, 94804, California
    Kevin J Scanlon

Authors

  1. Tadao Funato
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  2. Tomonori Ishii
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  3. Mariko Kambe
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  4. Kevin J Scanlon
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  5. Takeshi Sasaki
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Funato, T., Ishii, T., Kambe, M. et al. Anti-K-ras ribozyme induces growth inhibition and increased chemosensitivity in human colon cancer cells.Cancer Gene Ther 7, 495–500 (2000). https://doi.org/10.1038/sj.cgt.7700125

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