Inhibition of cell proliferation in HCC-9204 hepatoma cells by a c-myc specific ribozyme (original) (raw)

Cancer Gene Therapy volume 7, pages 407–412 (2000)Cite this article

Abstract

A ribozyme (RZ) gene targeting c-myc mRNA was synthesized and cloned. Cleavage reaction showed that cleavage of the RZ was efficient and specific. The RZ gene-containing retrovirus vector pDOR-RZ was transfected into HCC-9204 hepatoma cells, which constitutively express high levels of c-myc using Lipofectamine. Positively transfected cells were selected using G418. In situ hybridization showed that both pDOR-RZ and pDOR vectors had been integrated into the chromosome of HCC-9204 cells. Dot blot hybridization indicated that expression of the RZ was only evident in pDOR-RZ-transfected HCC-9204 cells. Avidin-biotin complex enzyme-linked immunosorbent assay showed that c-myc expression was down-regulated. Chromatin aggregation into compact masses, cytoplasmic vacuole degeneration, and blurring of cytoplasm structure were observed by transmission electron microscopy in HCC-9204-RZ cells. These results suggest that the use of a c-myc mRNA cleaving enzyme could be most effective in tumor cells that are highly proliferative and constitutively express high levels of c-myc.

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Authors and Affiliations

  1. Department of Pharmacology, New York University Medical Center, New York, NY 10016
    Jin Cheng & Arnold Stern
  2. Institute of Gastroenterology, The Second College of Xian Medical University, Xian, People's Republic of China
    Jin Cheng & Jinyan Luo
  3. Institute of Gastroenterology, The Xijing Hospital of The Fourth Military Medical University, Xian, People's Republic of China
    Xueyong Zhang & Jialu Hu
  4. Department of Biochemistry and Molecular-Biology, The Fourth Military Medical University, Xian, People's Republic of China
    Hongxiang Hui & Chengji Wang

Authors

  1. Jin Cheng
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  2. Jinyan Luo
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  3. Xueyong Zhang
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  4. Jialu Hu
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  5. Hongxiang Hui
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  6. Chengji Wang
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  7. Arnold Stern
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Corresponding author

Correspondence toArnold Stern.

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Cheng, J., Luo, J., Zhang, X. et al. Inhibition of cell proliferation in HCC-9204 hepatoma cells by a c-myc specific ribozyme.Cancer Gene Ther 7, 407–412 (2000). https://doi.org/10.1038/sj.cgt.7700127

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