HVJ (Sendai virus)-cationic liposomes: a novel and potentially effective liposome-mediated technique for gene transfer to the airway epithelium (original) (raw)

Gene Therapy volume 4, pages 631–638 (1997)Cite this article

Abstract

We designed a novel technique for targeted gene transfer into the airway epithelium. This was constructed using multilamellar cationic liposomes, containing N-(α-trimethylammonioacetyl)-didodecyl- D ox-glutamate chloride, phosphatidylcholine and cholesterol and fused with haemagglutinating virus of Japan (HVJ), namely HVJ cationic liposomes. Single aerosol delivery of this novel vector to the airway of rats led to a highly efficient and widespread transduction of fluorescein isothiocyanate-labeled oligonucleotides or lacZ gene into the bronchial epithelium and alveolar macrophages, but not into the alveolar epithelium. The efficiency of gene transfer to the airway epithelium with a single administration of the lacZ gene was about 47.6% in the trachea, 39.0% in the bronchi and proximal bronchioli, and 2.9% in the terminal bronchioli, respectively (mean value, n = 6). Expression level of the luciferase gene delivered with this novel system was much higher than that without HVJ, in both the trachea and lung tissue. Two pretreatment HVJ-cationic liposome vehicles every other week resulted in minimal inflammatory infiltration in the subepithelial layer with no significant reduction in efficiency of the following gene transfer. We propose that this novel HVJ cationic liposome-mediated gene transfer system may be suitable for clinical gene therapy to treat subjects with lethal lung diseases such as cystic fibrosis.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 6 print issues and online access

$259.00 per year

only $43.17 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Pathology I, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Y Yonemitsu, T Yoshizumi & K Sueishi
  2. Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Y Yonemitsu, T Yoshizumi & K Sugimachi
  3. Institute for Molecular and Cellular Biology, Osaka University, Japan
    Y Kaneda
  4. Third Department of Internal Medicine, Kurume University School of Medicine, Kurume, Japan
    A Muraishi

Authors

  1. Y Yonemitsu
    You can also search for this author inPubMed Google Scholar
  2. Y Kaneda
    You can also search for this author inPubMed Google Scholar
  3. A Muraishi
    You can also search for this author inPubMed Google Scholar
  4. T Yoshizumi
    You can also search for this author inPubMed Google Scholar
  5. K Sugimachi
    You can also search for this author inPubMed Google Scholar
  6. K Sueishi
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Yonemitsu, Y., Kaneda, Y., Muraishi, A. et al. HVJ (Sendai virus)-cationic liposomes: a novel and potentially effective liposome-mediated technique for gene transfer to the airway epithelium.Gene Ther 4, 631–638 (1997). https://doi.org/10.1038/sj.gt.3300463

Download citation

Keywords

This article is cited by