Efficient adenovirus-mediated gene transduction of normal and leukemic hematopoietic cells (original) (raw)

Gene Therapy volume 4, pages 1093–1099 (1997)Cite this article

Abstract

We evaluated the efficiency of adenovirus-mediated gene transfer into normal and malignant human hematopoietic cells. An E-1 and E-3 deleted, replication-defective recombinant Ad.RSVβgal vector was used and the transduction efficiency was studied at a multiplicity of infection of 13 p.f.u. per cell. Approximately 40–50% of normal monocytes were transduced, whereas purified normal resting T cells and B cells were resistant to infection. We showed that 50–80% of primary chronic myeloid leukemia cells (CML, n = 12) were efficiently transduced. In contrast to CML, successful transduction of resting primary chronic B lymphocytic leukemia cells required appropriate preactivation of targeted cells. A novel protocol for the efficient transduction of adenovirus into B-CLL cells was presented. We showed that anti-CD40 mAb or CD40 ligand acts in synergy with rhIL-4 to enable the transduction of approximately 50–75% of B-CLL cells (B-CLL, n = 6). Expression of β-galactosidase in transduced CML cells and B-CLL cells was detected for at least 15 days after transduction. The present studies underline the utility of adenovirus vectors for the construction of cytokine gene-modified tumor vaccines for the treatment of hematopoietic malignancies such as CML and B-CLL.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 6 print issues and online access

$259.00 per year

only $43.17 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Clinical Immunology, University Hospital, Biomedical Center, Uppsala University, Uppsala, Sweden
    MR Huang, A Kallin & TH Tötterman
  2. Department of Animal Physiology, Biomedical Center, Uppsala University, Uppsala, Sweden
    M Olsson
  3. Medical Genetics, Biomedical Center, Uppsala University, Uppsala, Sweden
    U Pettersson

Authors

  1. MR Huang
    You can also search for this author inPubMed Google Scholar
  2. M Olsson
    You can also search for this author inPubMed Google Scholar
  3. A Kallin
    You can also search for this author inPubMed Google Scholar
  4. TH Tötterman
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Huang, M., Olsson, M., Kallin, A. et al. Efficient adenovirus-mediated gene transduction of normal and leukemic hematopoietic cells.Gene Ther 4, 1093–1099 (1997). https://doi.org/10.1038/sj.gt.3300499

Download citation

Keywords

This article is cited by