Establishment and characterization of a megakaryoblast cell line with amplification of MLL (original) (raw)

• Original Manuscript
• Published: 24 July 1998

Megakaryocytopoiesis and MLL amplification

• SD Smith1,
• RL Moldwin1,
• M-M Lu1,
• L Giordano1,
• C Vignon2,
• Y Suto2,
• A Harden2,
• R Tomek2,
• T Veldman3,
• T Ried3,
• RA Larson2,
• MM Le Beau2,
• JD Rowley2 &
• …
• N Zeleznik-Le2

Leukemia volume 12, pages 1119–1127 (1998)Cite this article

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Abstract

A new cell line with megakaryoblastic features, designated UoC-M1, was established from the malignant cells of a 68-year-old patient with acute myeloid leukemia. The patient’s leukemic cells reacted with α-naphthyl acetate esterase and acid phosphatase and expressed CD7, CD24, CD34, CD38, CD45, HLA-DR and CD61. Cytogenetic analysis of the patient’s malignant cells (and of the UoC-M1 cells) showed a human, male hypodiploid karyotype with many chromosome rearrangements and marker chromosomes. Spectral karyotyping (SKY) analysis complemented the G-banded karyotyping and clarified several chromosomal translocations and identified the marker chromosomes. Fluorescence in situ hybridization (FISH) and SKY analysis demonstrated that one marker chromosome contained three segments of chromosome 9 interspersed with three segments of chromosome 11, as well as a portion of chromosome 19. FISH analysis with a probe for MLL revealed that the UoC-M1 cells contained four copies of the MLL gene. Southern blot analysis determined that the MLL gene had a germline profile while Northern and Western analyses showed that the MLL mRNAs and protein were of the appropriate sizes. This is the first report of amplification of the MLL gene which may be an additional mechanism of leukemogenesis or disease progression.

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Authors and Affiliations

1. Department of Pediatrics, Section of Pediatric Hematology/Oncology, University of Chicago, IL, USA
   RJ Allen, SD Smith, RL Moldwin, M-M Lu & L Giordano
2. Department of Medicine, Section of Hematology/Oncology, University of Chicago, IL, USA
   C Vignon, Y Suto, A Harden, R Tomek, RA Larson, MM Le Beau, JD Rowley & N Zeleznik-Le
3. National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
   T Veldman & T Ried

Authors

1. RJ Allen
2. SD Smith
3. RL Moldwin
4. M-M Lu
5. L Giordano
6. C Vignon
7. Y Suto
8. A Harden
9. R Tomek
10. T Veldman
11. T Ried
12. RA Larson
13. MM Le Beau
14. JD Rowley
15. N Zeleznik-Le

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Allen, R., Smith, S., Moldwin, R. et al. Establishment and characterization of a megakaryoblast cell line with amplification of MLL.Leukemia 12, 1119–1127 (1998). https://doi.org/10.1038/sj.leu.2401002

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• Received: 04 September 1997
• Accepted: 21 January 1998
• Published: 24 July 1998
• Issue date: 01 July 1998
• DOI: https://doi.org/10.1038/sj.leu.2401002

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