Impaired activating receptor expression pattern in natural killer cells from patients with multiple myeloma (original) (raw)

Leukemia volume 20, pages 732–733 (2006)Cite this article

Multiple myeloma (MM) is a hematological malignancy characterized by accumulation in the bone marrow of plasma cells that secrete a monoclonal immunoglobulin (Ig). The implication of natural killer cells (NK) in antitumor immunity against MM is suggested by plasmocyte susceptibility to NK lysis.1 NK efficiently kill early-stage MM cells by a natural cytotoxicity receptor (NCR)- and NKG2D-dependent pathway.2 In contrast, late-stage MM cells are protected from NK lysis by a high expression of HLA class I molecules.2 In addition to this immune evasion mechanism involving the target cell,2 we tested the hypothesis of an additional mechanism involving the effector cell, that is, NK, as previously described in leukemic patients.3

We analyzed peripheral NK from six MM patients at diagnosis. The activating receptor expression was checked on CD19−CD3−CD56+ cells by flow cytometry using mAbs against NKp30, NKp44, NKp46, NKG2D, CD16 and 2B4/CD244 (for patient descriptions, see Supplementary Information).

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References

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Acknowledgements

This work was supported by Groupement entreprise française lutte cancer, association pour la recherche contre le cancer, Fédération nationale des centres de lutte contre le cancer, Fondation pour la Recherche Médicale.

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Authors and Affiliations

  1. Laboratoire d'Immunologie des Tumeurs INSERM U599, Institut Paoli-Calmettes, Marseille, France
    C Fauriat, F Mallet, D Olive & R T costello
  2. Département d'Hématologie, Hôpital Nord, Marseille, France
    R T costello
  3. Université de la Méditerranée, Marseille, France
    D Olive & R T costello

Authors

  1. C Fauriat
  2. F Mallet
  3. D Olive
  4. R T costello

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Correspondence toC Fauriat.

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Fauriat, C., Mallet, F., Olive, D. et al. Impaired activating receptor expression pattern in natural killer cells from patients with multiple myeloma.Leukemia 20, 732–733 (2006). https://doi.org/10.1038/sj.leu.2404096

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