Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase (original) (raw)

• Original Paper
• Published: 30 January 1997

Oncogene volume 14, pages 481–490 (1997)Cite this article

• 634 Accesses
• 199 Citations
• 6 Altmetric
• Metrics details

Abstract

Manganese superoxide dismutase (MnSOD) has been previously shown to suppress the malignant phenotype of human melanoma and breast cancer cells. To test the possible role of MnSOD in glioma malignancy, MnSOD was overexpressed in wild type human glioma U118 cells and subcloned U118-9 cells by transfection of human MnSOD cDNA. The MnSOD-transfected cell lines demonstrated expression of exogenous (plasmid) MnSOD mRNA, increase in MnSOD immunoreactive protein, and a three- to eightfold increase in MnSOD enzymatic activity. The MnSOD overexpressing cell lines became less malignant as demonstrated by requiring a higher serum concentration to grow in vitro and much slower tumor growth in nude mice than the parental and neo control cell lines. These findings further support the hypothesis that MnSOD may be a tumor suppressor gene in a wide variety of human tumors.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 50 print issues and online access

$259.00 per year

only $5.18 per issue

Buy this article

• Purchase on SpringerLink
• Instant access to the full article PDF.

USD 39.95

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

• Log in
• Learn about institutional subscriptions
• Read our FAQs
• Contact customer support

Similar content being viewed by others

Author information

Authors and Affiliations

1. Radiation Research Laboratory, 77 Medical Laboratories, The University of Iowa, Iowa City, 52242, Iowa, USA
   Weixiong Zhong & Larry W Oberley
2. Pathology Service, William S Middleton Veterans Memorial Hospital,
   Terry D Oberley
3. Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, 53705, Wisconsin, USA
   Terry D Oberley
4. Graduate Center for Toxicology, University of Kentucky, Lexington, 40536, Kentucky, USA
   Daret K St Clair

Authors

1. Weixiong Zhong
2. Larry W Oberley
3. Terry D Oberley
4. Daret K St Clair

Rights and permissions

About this article

Cite this article

Zhong, W., Oberley, L., Oberley, T. et al. Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase.Oncogene 14, 481–490 (1997). https://doi.org/10.1038/sj.onc.1200852

Download citation

• Received: 06 June 1996
• Revised: 30 September 1996
• Accepted: 01 October 1996
• Issue date: 30 January 1997
• DOI: https://doi.org/10.1038/sj.onc.1200852

Keywords

This article is cited by