Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein (original) (raw)

Oncogene volume 14, pages 2425–2433 (1997)Cite this article

Abstract

We have cloned a cDNA for a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein, termed NE-dlg (**n**euronal and **e**ndocrine dlg). Northern blot analysis revealed that the gene is highly expressed in neuronal and endocrine tissues. Fluorescence in situ hybridization (FISH) and radiation hybrid mapping studies localized the NE-dlg gene to chromosome Xq13. We also found that the NE-dlg gene encoded a 100 kDa protein. Immunolocalization studies using an NE-dlg antibody showed that the protein tended to be expressed in non-proliferating cells, such as neurons, cells in Langerhans islets of the pancreas, myocytes of the heart muscles, and the prickle and functional layer cells of the esophageal epithelium. Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little expression of the NE-dlg protein. In addition, yeast two-hybrid screening and in vitro binding assays revealed that the NE-dlg interacted with the carboxyl-terminal region of the APC tumor suppressor protein. These data suggest that NE-dlg negatively regulates cell proliferation through its interaction with the APC protein.

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Authors and Affiliations

  1. Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, 2-2-1 Honjo Kumamoto 860, Japan
    Keishi Makino, Hiroaki Kuwahara, Norio Masuko, Yasuyuki Nishiyama, Tetsurou Morisaki, Ji-ichiro Sasaki, Mitsuyoshi Nakao & Hideyuki Saya
  2. Department of Neurosurgery, Kumamoto University School of Medicine, 2-2-1 Honjo Kumamoto 860, Japan
    Keishi Makino & Yukitaka Ushio
  3. Department of Hygiene Ehime, University School of Medicine, Ehime, 791-02, Japan
    Akira Kuwano
  4. Biomedical R&D Department, Sumitomo Electric Industries, Ltd, Yokohama, 244, Japan
    Motomi Nakata

Authors

  1. Keishi Makino
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  2. Hiroaki Kuwahara
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  3. Norio Masuko
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  4. Yasuyuki Nishiyama
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  5. Tetsurou Morisaki
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  6. Ji-ichiro Sasaki
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  7. Mitsuyoshi Nakao
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  8. Akira Kuwano
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  9. Motomi Nakata
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  10. Yukitaka Ushio
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  11. Hideyuki Saya
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Makino, K., Kuwahara, H., Masuko, N. et al. Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein.Oncogene 14, 2425–2433 (1997). https://doi.org/10.1038/sj.onc.1201087

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