Identification and characterization of nucleophosmin/B23/numatrin which binds the anti-oncogenic transcription factor IRF-1 and manifests oncogenic activity (original) (raw)
- Original Paper
- Published: 11 September 1997
- Naoto Minamino2,
- Tokiko Nagamura-Inoue1,
- Masahito Matsumoto1,
- Tadatsugu Taniguchi1 &
- …
- Nobuyuki Tanaka1
Oncogene volume 15, pages 1275–1281 (1997)Cite this article
Abstract
Interferon regulatory factor-1 (IRF-1) acts as a transcriptional activator in the interferon system and as a tumor suppressor. The loss of functional IRF-1 has been observed in a significant number of patients with myelodysplastic syndrome (MDS) and leukemia, suggesting a potentially critical role of IRF-1 in human oncostasis. Here we report an alternative mechanism by which IRF-1 may be inactivated. We purified an IRF-1 association molecule which was revealed to be identical to a nuclear factor nucleophosmin (NPM)/B23/numatrin. Functional analysis showed that NPM inhibited the DNA-binding and transcriptional activity of IRF-1. Moreover, NPM was overexpressed in several clinical leukemia samples and human-derived leukemia cell lines. Finally, overexpression of NPM in NIH3T3 cells resulted in malignant transformation. These results suggest the possible involvement of NPM in inactivating IRF-1-dependent anti-oncogenic surveillance in human cancer development.
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Authors and Affiliations
- Department of Immunology, Faculty of Medicine, Tokyo University, Hongo 7-3-1, Bunkyo-ku, 113, Tokyo
Takeshi Kondo, Tokiko Nagamura-Inoue, Masahito Matsumoto, Tadatsugu Taniguchi & Nobuyuki Tanaka - National Cardiovascular Center Research Institute, Fujishirodai 5, Suita-shi, 565, Osaka, Japan
Naoto Minamino
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- Takeshi Kondo
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Kondo, T., Minamino, N., Nagamura-Inoue, T. et al. Identification and characterization of nucleophosmin/B23/numatrin which binds the anti-oncogenic transcription factor IRF-1 and manifests oncogenic activity.Oncogene 15, 1275–1281 (1997). https://doi.org/10.1038/sj.onc.1201286
- Received: 09 January 1997
- Revised: 16 May 1997
- Accepted: 16 May 1997
- Issue Date: 11 September 1997
- DOI: https://doi.org/10.1038/sj.onc.1201286