Expression of the neoplastic phenotype by human thyroid carcinoma cell lines requires NFκB p65 protein expression (original) (raw)

• Original Paper
• Published: 16 October 1997
• Janete Cerutti1 na1,
• Sabrina Battista2,
• Monica Fedele2,
• Francesco Trapasso3,
• Kazuya Zeki4,
• Maria Pia Miano2,
• Filomena de Nigris2,
• Laura Casalino5,
• Francesco Curcio6,
• Massimo Santoro1 &
• …
• Alfredo Fusco3

Oncogene volume 15, pages 1987–1994 (1997)Cite this article

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Abstract

We have investigated the role of the NFκB complex in the process of thyroid carcinogenesis by analysing thyroid carcinoma cell lines. A significant increase in p65 NFκB mRNA and protein expression, compared to normal thyroid cultures or tissue, was found in all of the cancer cell lines. Conversely, only a modest increase in the p50 NFκB mRNA and protein was found in most, but not all carcinoma cell lines. The block of p65 protein synthesis with specific antisense oligonucleotides greatly reduced the ability of two undifferentiated carcinoma cell lines to form colonies in agar and reduced their growth rate. On the other hand, no effect was observed in the same cell lines when treated with p50 specific antisense oligonucleotides. These inhibitory effects seem to be mediated by the suppression of c-myc gene expression, since treatment with antisense oligonucleotides for p65 gene interfered negatively with c-myc gene expression. Our results indicate that activation of the NFκB complex by overexpression of p65 plays a critical role in the process of thyroid cell transformation.

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Author notes

1. Roberta Visconti and Janete Cerutti: The first two authors have equally contributed to this work

Authors and Affiliations

1. Dipartimento di Biologia e Patologia Cellulare e Molecolare c/o Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli 'Federico II', via Pansini, 5, Napoli, 80131, Italy
   Roberta Visconti, Janete Cerutti & Massimo Santoro
2. Istituto Nazionale dei Tumori di Napoli, via Mariano Semmola, Napoli, 80131, Italy
   Sabrina Battista, Monica Fedele, Maria Pia Miano & Filomena de Nigris
3. Dipartimento di Medicina Sperimentale e Clinica, Facoltà di Medicina e Chirurgia di Catanzaro, Università di Reggio Calabria, via Tommaso Campanella, Catanzaro, 88100, Italy
   Francesco Trapasso & Alfredo Fusco
4. The First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, 807, Kitakyushu, Japan
   Kazuya Zeki
5. Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, via Guglielmo Marconi, 10, Napoli, 80125, Italy
   Laura Casalino
6. Dipartimento di Patologia e Medicina Sperimentale e Clinica, Università degli Studi di Udine, Udine, Italy
   Francesco Curcio

Authors

1. Roberta Visconti
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2. Janete Cerutti
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3. Sabrina Battista
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4. Monica Fedele
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5. Francesco Trapasso
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6. Kazuya Zeki
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7. Maria Pia Miano
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8. Filomena de Nigris
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9. Laura Casalino
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10. Francesco Curcio
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11. Massimo Santoro
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12. Alfredo Fusco
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Visconti, R., Cerutti, J., Battista, S. et al. Expression of the neoplastic phenotype by human thyroid carcinoma cell lines requires NFκB p65 protein expression.Oncogene 15, 1987–1994 (1997). https://doi.org/10.1038/sj.onc.1201373

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• Received: 10 February 1996
• Revised: 24 June 1997
• Accepted: 25 June 1997
• Issue Date: 16 October 1997
• DOI: https://doi.org/10.1038/sj.onc.1201373

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