Identification of PTEN/MMAC1 alterations in uncultured melanomas and melanoma cell lines (original) (raw)

Oncogene volume 16, pages 3397–3402 (1998)Cite this article

Abstract

A novel tumor suppressor gene, PTEN/MMAC1, has been recently shown to be mutated in gliomas, breast, prostate, kidney cancers and melanomas. Loss-of-heterozygosity studies in melanoma have suggested the presence of at least one chromosome 10q locus lost early in tumor progression. In this study, we screened 45 melanoma cell lines and 17 paired uncultured metastatic melanoma and peripheral blood specimens for PTEN/MMAC1 alterations using PCR-SSCP and direct sequencing. We found nine melanoma cell lines with homozygous deletions (five with intragenic loss) and four cell lines with mutations (one nonsense and one frameshift; two intronic); from among our uncultured melanoma specimens, we found one tumor with a somatic 17 bp duplication in exon 7 leading to a premature stop codon and one tumor with a possible homozygous deletion. Furthermore, we have identified a novel intragenic polymorphism within intron 4 of PTEN/MMAC1. Taken together, these data suggest that PTEN/MMAC1 may be a chromosome 10q tumor suppressor important in melanoma tumor formation or progression.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 50 print issues and online access

$259.00 per year

only $5.18 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Dermatology, Massachusetts General Hospital and Dana Farber/Partners CancerCare, Fruit Street, Boston, 02114, Massachusetts, USA
    Hensin Tsao
  2. Division of Hematology/Oncology, Massachusetts General Hospital and Dana Farber/Partners CancerCare, Fruit Street, Boston, 02114, Massachusetts, USA
    Hensin Tsao, Xue Zhang, Eric Benoit & Frank G Haluska

Authors

  1. Hensin Tsao
    You can also search for this author inPubMed Google Scholar
  2. Xue Zhang
    You can also search for this author inPubMed Google Scholar
  3. Eric Benoit
    You can also search for this author inPubMed Google Scholar
  4. Frank G Haluska
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Tsao, H., Zhang, X., Benoit, E. et al. Identification of PTEN/MMAC1 alterations in uncultured melanomas and melanoma cell lines.Oncogene 16, 3397–3402 (1998). https://doi.org/10.1038/sj.onc.1201881

Download citation

Keywords

This article is cited by