Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteins (original) (raw)

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Published: 14 July 1998

Oncogene volume 16, pages 3413–3421 (1998)Cite this article

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Abstract

Several proteins that may regulate c-myc mRNA post-transcriptionally were previously isolated and characterized. Two of them, HuR and AUF1, bind specifically to the 3′ untranslated region (UTR) of c-myc mRNA. Because c-myc is regulated post-transcriptionally in various mouse tissues, including quiescent tissues, fetal liver and regenerating liver, we investigated whether HuR and AUF1 expression was also regulated in these tissues. Concerning AUF1, we analysed the expression of various mRNA and protein isoforms. We discovered a new AUF1 mRNA variant with a long AU-rich 3′ UTR. We show that AUF1 expression, regardless of the RNA isoform considered, and HuR mRNA expression parallel c-myc expression in quiescent tissues and during liver development; their expression is high in lymphoid tissues and fetal liver and low in adult liver. However, no up-regulation of HuR or AUF1 accompanies the up-regulation of c-myc mRNA following partial hepatectomy. We discuss our results in relation to the current hypothesis that HuR and AUF1 act as mRNA destabilizing factors.

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Authors and Affiliations

Centre de Biologie du Développement, CNRS-UMR 5547, Université Paul Sabatier, 118 Rte de Narbonne, Toulouse, Cedex 04 31062, France
Isabelle Lafon, Fabrice Carballès, Maryse Poiret & Dominique Morello
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, 27157-1064, North Carolina, USA
Gary Brewer

Authors

Isabelle Lafon
Fabrice Carballès
Gary Brewer
Maryse Poiret
Dominique Morello

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Lafon, I., Carballès, F., Brewer, G. et al. Developmental expression of AUF1 and HuR, two c-myc mRNA binding proteins.Oncogene 16, 3413–3421 (1998). https://doi.org/10.1038/sj.onc.1201895

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Received: 15 December 1997
Revised: 02 February 1998
Accepted: 03 February 1998
Published: 14 July 1998
Issue date: 02 July 1998
DOI: https://doi.org/10.1038/sj.onc.1201895