Modulation of endoplasmic reticulum calcium pump by Bcl-2 (original) (raw)

Oncogene volume 17, pages 1903–1910 (1998)Cite this article

Abstract

Members of the bcl-2 gene family encode proteins that function either to promote or to inhibit apoptosis. Despite numerous efforts, the mechanism of action of Bcl-2, an anti-apoptotic protein, is still not clear. In particular, the relation between Bcl-2 and the endoplasmic reticulum (ER) calcium store is not well-understood. In the present work, we examined the effect of Bcl-2 on the ER store. We demonstrate that overexpression of Bcl-2 in breast epithelial cells modulates ER store by upregulating calcium pump (SERCA) expression without affecting the release channel (IP3R). The steady state levels of SERCA2 mRNA and protein were both increased in Bcl-2 expression clones. The increase in SERCA2 protein leads to accelerated calcium uptake and enhanced Ca2+ loading. In addition, we also show the detection of intracellular interaction between Bcl-2 and SERCA molecules by co-immunoprecipitation. Since high lumenal Ca2+ concentration of ER is essential for normal cell functions, the results suggest that Bcl-2 preserves the ER Ca2+ store by upregulating SERCA gene expression as well as by a possible interaction with the pump.

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Authors and Affiliations

  1. Department of Pathology, Wayne State University School of Medicine, Detroit, 48201, Michigan, USA
    Tuan H Kuo, Hyeong-Reh Choi Kim, Liping Zhu, Yingjie Yu, Huei-Min Lin & Wayne Tsang

Authors

  1. Tuan H Kuo
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  2. Hyeong-Reh Choi Kim
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  3. Liping Zhu
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  4. Yingjie Yu
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  5. Huei-Min Lin
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  6. Wayne Tsang
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Kuo, T., Kim, HR., Zhu, L. et al. Modulation of endoplasmic reticulum calcium pump by Bcl-2.Oncogene 17, 1903–1910 (1998). https://doi.org/10.1038/sj.onc.1202110

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