Differential regulation of cellular target genes by p53 devoid of the PXXP motifs with impaired apoptotic activity (original) (raw)

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• Published: 08 April 1999

Oncogene volume 18, pages 2149–2155 (1999)Cite this article

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Abstract

Activation of the p53 tumor suppressor protein can lead to either cell cycle arrest or apoptosis. Several functional domains necessary for mediating cell cycle arrest and apoptosis in p53 have been mapped, e.g., the proline-rich domain. The proline-rich domain is located within residues 60 – 90, which comprise five PXXP motifs (where P represents proline and X any amino acid). To further delineate the function of the proline-rich domain and its potential role in transactivation, we generated several groups of cell lines that inducibly express various p53 mutants using a tetracycline-regulated expression system. We found that p53(Δ62 – 91), which lacks all five PXXP motifs in human p53, is capable of inducing cell cycle arrest but not apoptosis, while p53(gln22-ser23/Δ62 – 91), which contains a double point mutation in the activation domain as well as deletion of the proline-rich domain, completely loses its activity. However, p53(Δ74 – 91), which contains only one PXXP motif at its N-terminus, is not only capable of inducing cell cycle arrest but also retains a partial apoptotic activity. Furthermore, we found that deletion of the proline-rich region has no or very mild effects on activation of several transiently transfected p53 target gene promoters, i.e., the p21, MDM2, BAX, and GADD45 promoters. However, such deletion differentially affects p53 induction of endogenous target genes, i.e., induction of p21, MDM2, BTG2, p85, PIG3, PIG6 and PIG11 was reduced or abrogated but induction of BAX, KILLER/DR5, PIG2, PIG7 and PIG8 was not substantially affected. Interestingly, induction of GADD45 was enhanced. These results suggest that the proline-rich region may play a role in chromatin remodeling, which counteracts chromatin-mediated repression for some of the endogenous p53 target genes.

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Acknowledgements

We thank Rhea Markowitz, William Dynan, Mark Anderson, and Pat Schoenlein for critical reading of this manuscript and for helpful discussions and suggestions. This work was supported in part by grants from the United States Department of Defense Army Breast Cancer Program DAMD17-97-I-7019 and the National Institute of Health CA76069.

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Author notes

1. Jianhui Zhu and Jieyuan Jiang: J Zhu and J Jiang contributed equally to this work

Authors and Affiliations

1. Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, 30912, Georgia, USA
   Jianhui Zhu, Jieyuan Jiang, Wenjing Zhou, Kuichun Zhu & Xinben Chen

Authors

1. Jianhui Zhu
2. Jieyuan Jiang
3. Wenjing Zhou
4. Kuichun Zhu
5. Xinben Chen

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Zhu, J., Jiang, J., Zhou, W. et al. Differential regulation of cellular target genes by p53 devoid of the PXXP motifs with impaired apoptotic activity.Oncogene 18, 2149–2155 (1999). https://doi.org/10.1038/sj.onc.1202533

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• Received: 21 January 1998
• Revised: 27 October 1998
• Accepted: 27 October 1998
• Published: 08 April 1999
• Issue date: 25 March 1999
• DOI: https://doi.org/10.1038/sj.onc.1202533

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