Purification of recombinant proteins by fusion with thermally-responsive polypeptides (original) (raw)

References

  1. Nilsson, B., Forsberg, G., Moks, T., Hartmanis, M. & Uhlén, M. Fusion proteins in biotechnology and structural biology. Curr. Opin. Struct. Biol. 2, 569–575 (1992).
    Article CAS Google Scholar
  2. Uhlén, M. & Moks, T. Gene fusions for purpose of expression: an introduction. Methods Enzymol. 195, 129–143 (1990).
    Article Google Scholar
  3. Maina, C.V. et al. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose binding protein. Gene 74, 365–373 (1988).
    Article CAS Google Scholar
  4. Smith, D.B. & Johnson, K.S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31–40 (1988).
    Article CAS Google Scholar
  5. Tsao, K.W., deBarbieri, B., Hanspeter, M. & Waugh, D.W. A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli. Gene 169, 59–64 (1996).
    Article CAS Google Scholar
  6. Smith, P.A. et al. A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli. Nucleic Acids Res. 26, 1414–1420 (1998).
    Article CAS Google Scholar
  7. LaVallie, E.R. et al. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 11, 187–193 (1993).
    CAS PubMed Google Scholar
  8. Ong, E. et al. The cellulose-binding domains of cellulases: tools for biotechnology. Trends Biotechnol. 7, 239–243 (1989).
    Article CAS Google Scholar
  9. Smith, M.C., Furman, T.C., Ingolia, T.D. & Pidgeon, C. Chelating peptide-immobilized metal ion affinity chromatography. J. Biol. Chem. 263, 7211–7215 (1988).
    CAS PubMed Google Scholar
  10. Kim, J-S. & Raines, R.T. Ribonuclease S-peptide as a carrier in fusion proteins. Protein. Sci. 2, 348–356 (1993).
    Article CAS Google Scholar
  11. Su, X., Prestwood, A.K. & McGraw, R.A. Production of recombinant porcine tumor necrosis factor alpha in a novel E. coli expression system. Biotechniques 13, 756–762 (1992).
    CAS PubMed Google Scholar
  12. Nilsson, J., Ståhl, S., Lundeberg, J., Uhlén, M. & Nygren, P.Å. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr. Purif. 11, 1–16 (1997).
    Article CAS Google Scholar
  13. Urry, D.W. Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition due to internal chain dynamics. J. Protein. Chem. 7, 1–34 (1988).
    Article CAS Google Scholar
  14. Urry, D.W. Free energy transduction in polypeptides and proteins based on inverse temperature transitions. Prog. Biophys. Mol. Biol. 57, 23–57 (1992).
    Article CAS Google Scholar
  15. Urry, D.W. Physical chemistry of biological free energy transduction as demonstrated by elastic protein-based polymers. J. Phys. Chem. B 101, 11007–11028 (1997).
    Article CAS Google Scholar
  16. McPherson, D.T., Xu, J. & Urry, D.W. Product purification by reversible phase transition following Escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP. Protein Expr. Purif. 7, 51–57 (1996).
    Article CAS Google Scholar
  17. Hoffman. A.S. Applications of thermally-reversible polymers and hydrogels in therapeutics and diagnostics. J. Controlled Release 6, 297–305 (1987).
    Article CAS Google Scholar
  18. Chen, J.P. & Hoffman A.S. Protein-polymer conjugates II. Affinity precipitation separation of immunogammaglobulin by a poly(_N_-isopropylacrylamide)-protein A conjugate. Biomaterials 11, 631–634 (1990).
    Article CAS Google Scholar
  19. Chilkoti, A., Chen, G-H., Stayton, P.S. & Hoffman, A.S. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Bioconjugate Chem. 5, 504–507 (1994).
    Article CAS Google Scholar
  20. Urry, D.W. et al. Temperature of polypeptide inverse temperature transition depends on mean residue hydrophobicity. J. Am. Chem. Soc. 113, 4346–4348 (1991).
    Article CAS Google Scholar
  21. Urry, D.W., Trapane, T.L. & Prasad, K.U. Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studies. Biopolymers 24, 2345–2356 (1985).
    Article CAS Google Scholar
  22. Vertesy, L., Oeding, V., Bender, R., Zepf, K. & Nesemann, G. Tendamistat (HOE 467), a tight-binding alpha-amylase inhibitor from Streptomyces tendae 4158. Eur. J. Biochem. 141, 505–512 (1984).
    Article CAS Google Scholar
  23. Porath, J. Immobilized metal ion affinity chromatography. Prot. Expr. Purif. 3, 262–282 (1992).
    Article Google Scholar
  24. Coligan, J.E., Dunn, B.M., Ploegh, H.L., Speicher, D.W. & Wingfield, P.T. Current protocols in protein science. (John Wiley & Sons, New York; 1995).
    Google Scholar
  25. Hartmeier, W. Immobilized biocatalysts. (Springer-Verlag, Berlin; 1988).
    Book Google Scholar
  26. Diamandis, E.P. & Christopoulos, T.K. Immunoassay. (Academic Press, San Diego, CA; 1996).
    Google Scholar
  27. Dewhirst, M.W. & Samulski, T.V. Current Concepts: Hyperthermia in the treatment of cancer. (The UpJohn Co., Kalamazoo, MI; 1998).
    Google Scholar
  28. Hauck, M.L., Dewhirst, M.W., Bigner, D.D. & Zalutsky, M.R. Local hyperthermia improves uptake of a chimeric monoclonal antibody in a subcutaneous xenograft model. Clin. Cancer Res. 3, 63–70 (1997).
    CAS PubMed Google Scholar
  29. Cope, D.A., Dewhirst, M.W., Friedman, H.S., Bigner, D.D. & Zalutsky, M.R. Enhanced delivery of a monoclonal antibody F(ab′)2 fragment to subcutaneous human glioma xenografts using local hyperthermia. Cancer Res. 50, 1803–1809 (1990).
    CAS PubMed Google Scholar
  30. Ausubel, F.M. et al. Current protocols in molecular biology (John Wiley & Sons, New York; 1995).
    Google Scholar
  31. Holmgren, A. & Bjornstedt, M. Enzymatic reduction-oxidation of protein disulfides by thioredoxin. Methods Enzymol. 107, 295–300 (1984).
    Article CAS Google Scholar

Download references