Extraction of Nucleic Acid-free Lipopolysaccharides from Gram-negative Bacteria (original) (raw)
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- Published: 22 April 1961
Nature volume 190, pages 344–345 (1961)Cite this article
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Abstract
THE extraction of Gram-negative bacteria by the hot phenol method of Westphal1 yields a lipopolysaccharide along with considerable amounts of nucleic acid. The nucleic acid can be separated from the high molecular weight lipopolysaccharide by high-speed centrifugation, but to reduce the nucleic acid to a level of less than 2 per cent may require four or five sedimentations.
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References
- Westphal, O., Lüderitz, O., and Bister, F., Z. Naturforsch., 7, 148 (1952).
Article Google Scholar - Webster, Marion E., Sagin, J. F., Landy, M., and Johnson, A. G., J. Immunol., 74, 455 (1955).
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Authors and Affiliations
- Department of Biochemistry, School of Pharmacy, Royal College of Science and Technology, Glasgow
G. J. O'NEILL & J. P. TODD
Authors
- G. J. O'NEILL
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O'NEILL, G., TODD, J. Extraction of Nucleic Acid-free Lipopolysaccharides from Gram-negative Bacteria.Nature 190, 344–345 (1961). https://doi.org/10.1038/190344b0
- Issue Date: 22 April 1961
- DOI: https://doi.org/10.1038/190344b0