Extraction of Nucleic Acid-free Lipopolysaccharides from Gram-negative Bacteria (original) (raw)

Nature volume 190, pages 344–345 (1961)Cite this article

Abstract

THE extraction of Gram-negative bacteria by the hot phenol method of Westphal1 yields a lipopolysaccharide along with considerable amounts of nucleic acid. The nucleic acid can be separated from the high molecular weight lipopolysaccharide by high-speed centrifugation, but to reduce the nucleic acid to a level of less than 2 per cent may require four or five sedimentations.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Similar content being viewed by others

References

  1. Westphal, O., Lüderitz, O., and Bister, F., Z. Naturforsch., 7, 148 (1952).
    Article Google Scholar
  2. Webster, Marion E., Sagin, J. F., Landy, M., and Johnson, A. G., J. Immunol., 74, 455 (1955).
    CAS PubMed Google Scholar

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, School of Pharmacy, Royal College of Science and Technology, Glasgow
    G. J. O'NEILL & J. P. TODD

Authors

  1. G. J. O'NEILL
    You can also search for this author inPubMed Google Scholar
  2. J. P. TODD
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

O'NEILL, G., TODD, J. Extraction of Nucleic Acid-free Lipopolysaccharides from Gram-negative Bacteria.Nature 190, 344–345 (1961). https://doi.org/10.1038/190344b0

Download citation

This article is cited by