Origin of Small Lymphocytes following Blastogenesis induced by Short-term PHA Stimulation (original) (raw)

Nature volume 225, pages 857–858 (1970)Cite this article

Abstract

WE have reported that short-term (24 h) exposure of human lymphocyte cultures to phytohaemagglutinin (PHA) results in the conversion of 90 per cent or more of the small lymphocytes to “blastlike” forms. Subsequently, the cultures revert to cells which resemble the original unstimulated small lymphocytes, both morphologically and metabolically, but remain fully reactive to renewed PHA stimulation1. In this report, we offer evidence that, in the 24 h PHA-stimulated cultures, most lymphocytes become blastoid but do not divide; instead, they return to their original form by 9 or 10 days after stimulation.

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References

  1. Polgar, P. R., Kibrick, S., and Foster, J. M., Nature, 218, 596 (1968).
    Article ADS CAS Google Scholar
  2. Polgar, P. R., Foster, J. M., and Cooperband, S. R., Exp. Cell Res., 49, 231 (1968).
    Article CAS Google Scholar
  3. Phillips, H. J., and Andrews, R. V., Exp. Cell Res., 16, 678 (1959).
    Article CAS Google Scholar
  4. Kopriwa, B., and Lebland, C. P., J. Histochem. Cytochem., 10, 269 (1962).
    Article CAS Google Scholar
  5. Polgar, P. R., Cooperband, S. R., and Kibrick, S., Proc. Fourth Leukocyte Culture Conference (1969).
    Google Scholar
  6. Razavi, L., Nature, 210, 444 (1966).
    Article ADS CAS Google Scholar
  7. Canard, R. A., Nature, 214, 709 (1967).
    Article ADS Google Scholar

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Authors and Affiliations

  1. Departments of Biochemistry, Microbiology and Medicine, Boston University School of Medicine, Boston University Medical Center, Boston, Massachusetts
    PETER R. POLGAR & SIDNEY KIBRICK

Authors

  1. PETER R. POLGAR
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  2. SIDNEY KIBRICK
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POLGAR, P., KIBRICK, S. Origin of Small Lymphocytes following Blastogenesis induced by Short-term PHA Stimulation.Nature 225, 857–858 (1970). https://doi.org/10.1038/225857a0

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