Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast (original) (raw)

• Article
• Published: 04 July 1970
• H. BÜCHI1,
• M. H. CARUTHERS1,
• N. GUPTA1,
• H. G. KHORANA1,
• K. KLEPPE1,
• A. KUMAR1,
• E. OHTSUKA1,
• U. L. RAJBHANDARY1,
• J. H. VAN DE SANDE1,
• V. SGARAMELLA1,
• H. WEBER1 &
• …
• T. YAMADA 1

Nature volume 227, pages 27–34 (1970)Cite this article

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Abstract

By exploiting the natural ability of polynucleotides to align by base pairing and using polynucleotide kinase and ligase, chemically synthesized segments have been combined into a double stranded DNA corresponding to the gene for the earliest characterized tRNA.

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Authors and Affiliations

1. Institute for Enzyme Research, University of Wisconsin, Madison, Wisconsin, 53706
   K. L. AGARWAL, H. BÜCHI, M. H. CARUTHERS, N. GUPTA, H. G. KHORANA, K. KLEPPE, A. KUMAR, E. OHTSUKA, U. L. RAJBHANDARY, J. H. VAN DE SANDE, V. SGARAMELLA, H. WEBER & T. YAMADA

Authors

1. K. L. AGARWAL
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2. H. BÜCHI
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3. M. H. CARUTHERS
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4. N. GUPTA
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5. H. G. KHORANA
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6. K. KLEPPE
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7. A. KUMAR
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8. E. OHTSUKA
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9. U. L. RAJBHANDARY
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10. J. H. VAN DE SANDE
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11. V. SGARAMELLA
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12. H. WEBER
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13. T. YAMADA
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AGARWAL, K., BÜCHI, H., CARUTHERS, M. et al. Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast .Nature 227, 27–34 (1970). https://doi.org/10.1038/227027a0

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• Received: 16 June 1970
• Issue Date: 04 July 1970
• DOI: https://doi.org/10.1038/227027a0