Chromosomal variation and the establishment of somatic cell lines in vitro (original) (raw)

• Letter
• Published: 31 January 1975

Nature volume 253, pages 361–362 (1975)Cite this article

• 50 Accesses
• 36 Citations
• Metrics details

Abstract

WHEN somatic cells from a number of species are explanted in vitro, they may either completely fail to grow, or they may continue to divide for a limited number of generations, at a progressively lower rate and with decreasing plating efficiency. They will eventually die unless, from this “crisis”, a new population emerges with altered properties (see for example ref. 1). This new population, the established line, is usually capable of existing indefinitely and its growth rate and plating efficiency progressively increase until they stabilise at a level characteristic of each individual line.

This is a preview of subscription content, access via your institution

Access options

Subscribe to this journal

Receive 51 print issues and online access

$199.00 per year

only $3.90 per issue

Buy this article

• Purchase on SpringerLink
• Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

• Log in
• Learn about institutional subscriptions
• Read our FAQs
• Contact customer support

Similar content being viewed by others

References

1. Todaro, G. J., and Green, H., J. Cell Biol., 17, 299–313 (1963).
   Article CAS Google Scholar
2. Martin, G. M., and Sprague, C. A., Science, 166, 761–763 (1969).
   Article ADS CAS Google Scholar
3. Goldstein, S., and Lin, C. C., Expl Cell Res., 70, 436–439 (1972).
   Article CAS Google Scholar
4. Weiss, M. C., and Green, H., Proc. natn. Acad. Sci. U.S.A., 58, 1104–1109 (1967).
   Article ADS CAS Google Scholar
5. Terzi, M., J. cell Physiol., 80, 359–366 (1972).
   Article CAS Google Scholar
6. Pera, F., and Rainer, B., Chromosoma, 42, 71–86 (1973).
   Article CAS Google Scholar
7. Defendi, V., and Stoker, M. G. P., Nature new Biol., 242, 24–26 (1973).
   Article CAS Google Scholar
8. Larizza, L., Simoni, G., Tredici, F., and De Carli, L., Mutat. Res., 25, 123–130 (1974).
   Article CAS Google Scholar
9. Diamond, L., Int. J. Cancer, 2, 143–152 (1967).
   Article CAS Google Scholar
10. Tsuda, T., Tohoku J. exp. Med., 86, 380–393 (1965).
    Article CAS Google Scholar
11. Popescu, N. C., and DiPaolo, J. A., Cytogenetics, 11, 500–507 (1972).
    Article CAS Google Scholar
12. Hughes, D. T., Nature, 217, 518–523 (1968).
    Article ADS CAS Google Scholar
13. Allderdice, P. W., et al., J. Cell Sci., 12, 263–274 (1973).
    CAS PubMed Google Scholar
14. Tanamura, A., and Yerganian, G., Genetics, 41, 664–665 (1956).
    Google Scholar
15. Ohno, S., In vitro, 2, 46–57 (1966).
    Article Google Scholar

Download references

Author information

Authors and Affiliations

1. Laboratorio di Mutagenesi e Differenziamento (CNR) Via Cisanello, 147–56100 Pisa, Italy
   M. TERZI & T. S. C. HAWKINS
2. Imperial Cancer Research Fund, London, WC2A 3PX, UK
   M. TERZI & T. S. C. HAWKINS

Authors

1. M. TERZI
   You can also search for this author inPubMed Google Scholar
2. T. S. C. HAWKINS
   You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

TERZI, M., HAWKINS, T. Chromosomal variation and the establishment of somatic cell lines in vitro.Nature 253, 361–362 (1975). https://doi.org/10.1038/253361a0

Download citation

• Received: 31 May 1974
• Revised: 05 December 1974
• Issue Date: 31 January 1975
• DOI: https://doi.org/10.1038/253361a0

This article is cited by